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Peripherin antibody

Anti-Peripherin antibody used in Western Blot (WB). GTX102005
Anti-Peripherin antibody used in IHC (Paraffin sections) (IHC-P). GTX102005

Cat. No. GTX102005

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Peripherin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

PC-3

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 40% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Peripherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1241173

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peripherin , NEF4 , PRPH1

Background

This gene encodes a cytoskeletal protein found in neurons of the peripheral nervous system. The encoded protein is a type III intermediate filament protein with homology to other cytoskeletal proteins such as desmin, and is a different protein that the peripherin found in photoreceptors. Mutations in this gene have been associated with susceptibility to amyotrophic lateral sclerosis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Peripherin antibody used in Western Blot (WB). GTX102005

GTX102005 WB Image

Sample (30 ug of whole cell lysate)
A: PC-3
10% SDS PAGE
GTX102005 diluted at 1:1000

Anti-Peripherin antibody used in IHC (Paraffin sections) (IHC-P). GTX102005

GTX102005 IHC-P Image

Peripherin antibody detects PRPH protein at cytosol on Ca922 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded Ca922 xenograft.
Peripherin antibody (GTX102005) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Bodnar-Wachtel M et al. Life Sci Alliance 2023; 6 (4) Inflammasome-independent NLRP3 function enforces ATM activity in response to genotoxic stress.
Application : WB
Reactivity : Human
Nakayama I et al. J Biochem 2020; (Epub) Regulation of Epidermal Growth Factor Receptor Expression and Morphology of Lung Epithelial Cells by Interleukin-1帣.
Application : ICC/IF
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169