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Periplakin antibody [N1N2], N-term

Anti-Periplakin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX117411
Anti-Periplakin antibody [N1N2], N-term used in Western Blot (WB). GTX117411
Anti-Periplakin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX117411

Cat. No. GTX117411

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all Periplakin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

205 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human Periplakin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177667

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

periplakin

Cellular Localization

Cell junction , desmosome , Cytoplasm , cytoskeleton , Cell membrane , Nucleus , Mitochondrion

Background

The protein encoded by this gene is a component of desmosomes and of the epidermal cornified envelope in keratinocytes. The N-terminal domain of this protein interacts with the plasma membrane and its C-terminus interacts with intermediate filaments. Through its rod domain, this protein forms complexes with envoplakin. This protein may serve as a link between the cornified envelope and desmosomes as well as intermediate filaments. AKT1/PKB, a protein kinase mediating a variety of cell growth and survival signaling processes, is reported to interact with this protein, suggesting a possible role for this protein as a localization signal in AKT1-mediated signaling. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Periplakin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX117411

GTX117411 IHC-P Image

Periplakin antibody [N1N2], N-term detects Periplakin protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Periplakin antibody [N1N2], N-term (GTX117411) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Periplakin antibody [N1N2], N-term used in Western Blot (WB). GTX117411

GTX117411 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
5% SDS PAGE
GTX117411 diluted at 1:5000

Anti-Periplakin antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX117411

GTX117411 IHC-P Image

Periplakin antibody [N1N2], N-term detects PPL protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Periplakin antibody [N1N2], N-term (GTX117411) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169