Mixing of reagent: To five ml buffer (reagent B) in a test tube, add one drop of concentrated Enhancing reagent (reagent E) mix well. This ready to use reagent is good for 4-6 hours. Procedure: 1.) After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated) and distilled/ deionized water. 2.) Add few drops of ready to use enhancing reagent for 1-2 minutes. 3.) Add few drops of ready to use AEC reagent, or DAB reagent, follow instructions as outlined for these chromogens.
Reagent B (Buffer): 250 ml Reagent E (conc. Enhancinig reagent): 3 ml
Keep as concentrated solution, aliquot and store at 4ºC. DO NOT FREEZE.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Peroxidase enzyme system is routinely used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This Peroxidase Enhancing system increases the level of sensitivity in peroxidase based chromogens (AEC and DAB). This product is used prior to addition of chromogens