APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 10% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The clone AT22E7 is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PRDX8 protein.
Purification
Protein A Purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
peroxiredoxin 6 , AOP2 , HELS128m , NSGPx , PRDX6 , PRX , aiPLA2 , p29 , Peroxiredoxin 6 , 1Cys
Cellular Localization
Cytoplasm,Lysosome,Cytoplasmic vesicle
Background
The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX57622 FACS Image
FACS analysis of HeLa cells using GTX57622 Peroxiredoxin 6 antibody. Cell Number: 1 x 10⁶ cells Red: Primary antibody Antibody amount: 2-5 μg
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GTX57622 WB Image
WB analysis of various samples using GTX57622 Peroxiredoxin 6 antibody. Lane 1 : Recombinant Human PRDX6 protein (50 ng) Lane 2 : HeLa whole cell lysate (40 μg) Lane 3 : 293T whole cell lysate (40 μg) Lane 4 : HepG2 whole cell lysate (40 μg) Lane 5 : U87-MG whole cell lysate (40 μg) Lane 6 : Ramos whole cell lysate (40 μg)
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GTX57622 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX57622 Peroxiredoxin 6 antibody. Blue: DAPI Green: Primary antibody Dilution: 1:100
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REFERENCE
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REVIEW
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