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Peroxiredoxin 6 antibody

KO/KD Orthogonal
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

Cat. No. GTX115262

Reference 6
5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse, Rat, milkfish
Package
100 μl ($399),
25 μl ($169)
See all Peroxiredoxin 6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

Mouse brain , HCT116

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Peroxiredoxin 6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620162

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxiredoxin 6 , 1-Cys , AOP2 , HEL-S-128m , NSGPx , PRX , aiPLA2 , p29

Cellular Localization

Cytoplasm , Lysosome , Cytoplasmic vesicle

Background

The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

Sample (30 μg of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX115262 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX115262 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Peroxiredoxin 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115262

GTX115262 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Peroxiredoxin 6(GTX115262) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

Orthogonal

The data was published in the journal Front Physiol in 2016. PMID: 27965586

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

Orthogonal

The data was published in the journal Front Physiol in 2016. PMID: 27965586

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

Orthogonal

The data was published in the journal J Biomed Sci in 2013. PMID: 24359494

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

Orthogonal

The data was published in the journal Front Physiol in 2016. PMID: 27965586

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

Orthogonal

The data was published in the journal Proteome Sci in 2013. PMID: 23866759

REFERENCE

Application Reference

Chang CH et al. Front Physiol 2016; 7 : 580 The Antioxidant Peroxiredoxin 6 (Prdx6) Exhibits Different Profiles in the Livers of Seawater- and Fresh Water-Acclimated Milkfish, Chanos chanos, upon Hypothermal Challenge.
Chen JY et al. J Biomed Sci 2013; 20 : 95 quercetin-induced cardioprotection against * cytotoxicity.
Application : WB
Reactivity : Rat
Li JM et al. Environ Mol Mutagen 2013; 54 (6) : 429-49 Hyaluronic acid-dependent protection against UVB-damaged human corneal cells.
Application : WB
Reactivity : Human
Chilla A et al. Mol Cell Proteomics 2013; 12 (7) : 1926-38 Proteomic identification of VEGF-dependent protein enrichment to membrane caveolar-raft microdomains in endothelial progenitor cells.
Application : WB
Reactivity : Human
Liu Z et al. Proteome Sci 2013; 11 (1) : 32 Proteomic analysis of the inhibitory effect of epigallocatechin gallate on lipid accumulation in human HepG2 cells.
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

Peroxiredoxin 6 antibody Cat. No. GTX115262

Rating
( Average 5 based on 5 users reviews)
Application
Western Blot(WB)
( Average 5 based on 3 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Date : Anonymous submitted on 23-Jan-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Primary Antibody : 1:1000, 4°C, >12Hr
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Date : Anonymous submitted on 17-Nov-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain tissue lysate
Blocking : Block Ace, 24°C, 0.5Hr
Primary Antibody : 1:3000, 15°C, 16Hr
Anti-Peroxiredoxin 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115262
Date : Anonymous submitted on 22-Jun-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HAP1 WT and PRDX6 KO cells
Blocking : 5% BSA, 5% goat serum, and 0.01% Triton X-100 in PBS, 30 min, RT
Fixation : 4% PFA in PBS, 15 min, RT
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in PBS, 10 min, RT
Primary Antibody : 1:1000 in 5% BSA, 5% goat serum, and 0.01% Triton X-100 in PBS, 4°C, > 12Hr
Lane Description :
Yellow dashed line : WT cells
Magenta dashed line : KO cells
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Peroxiredoxin 6 antibody used in Immunoprecipitation (IP). GTX115262
Date : Anonymous submitted on 22-Jun-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description :
SM : 10% starting material
UB : 10% unbound fraction
IP : IP antibody
Figure Note : 2.0μg of antibody to 500μl of PBS with 0.01% triton X-100, together with 30μl of protein A-Sepharose beads. One ml aliquots at 0.5 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Date : Anonymous submitted on 22-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and PRDX6 KO cells
Amount used : 25μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:1000 5% BSA in TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169