GeneTex
United States (US)

Peroxiredoxin 6 antibody

GTX115262_40275_WB_M_brain_18121410_196.jpg
GTX115262_40275_IFA_18121410_427.jpg
GTX115262_WB_18121410_137.jpg

Cat No. GTX115262

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

A549 , HeLa , HepG2 , HCT116 , mouse brain

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Concentration

0.89mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Peroxiredoxin 6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

1-Cys antibody, AOP2 antibody, KIAA0106 antibody, MGC46173 antibody, NSGPx antibody, PRX antibody, aiPLA2 antibody, p29 antibody, PRDX6 antibody, 1-Cys PRX antibody, acidic calcium-independent phospholipase A2 antibody, antioxidant protein 2 antibody, non-selenium glutathione peroxidase antibody, 24 kDa protein antibody, liver 2D page spot 40 antibody, 1-Cys peroxiredoxin antibody, red blood cells page spot 12 antibody, peroxiredoxin-6 antibody, peroxiredoxin 6 antibody

Cellular Localization

Cytoplasm (By similarity) , Lysosome (By similarity) , Cytoplasmic vesicle (By similarity)

Background

The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX115262_40275_WB_M_brain_18121410_196.jpg

GTX115262 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX115262 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX115262_40275_IFA_18121410_427.jpg

GTX115262 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Peroxiredoxin 6(GTX115262) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX115262_WB_18121410_137.jpg

GTX115262 WB Image

Sample (30 µg of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX115262 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Reference
SDS
Glycerol.pdf
Thimerosal.pdf