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Peroxiredoxin 6 antibody

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

Cat. No. GTX115262

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse, Rat, milkfish
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

Positive Control

Mouse brain , HCT116

Predict Reactivity

Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Peroxiredoxin 6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10620162

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxiredoxin 6 , 1-Cys , AOP2 , HEL-S-128m , NSGPx , PRX , aiPLA2 , p29

Cellular Localization

Cytoplasm , Lysosome , Cytoplasmic vesicle

Background

The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

Sample (30 μg of whole cell lysate)
A: HCT116
12% SDS PAGE
GTX115262 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX115262 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Peroxiredoxin 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115262

GTX115262 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Peroxiredoxin 6(GTX115262) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

The data was published in the journal Front Physiol in 2016. PMID: 27965586
The data was published in the journal Front Physiol in 2016. PMID: 27965586

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

The data was published in the journal Front Physiol in 2016. PMID: 27965586
The data was published in the journal Front Physiol in 2016. PMID: 27965586

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

The data was published in the journal J Biomed Sci in 2013. PMID: 24359494
The data was published in the journal J Biomed Sci in 2013. PMID: 24359494

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

The data was published in the journal Front Physiol in 2016. PMID: 27965586
The data was published in the journal Front Physiol in 2016. PMID: 27965586

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX115262

GTX115262 WB Image

The data was published in the journal Proteome Sci in 2013. PMID: 23866759
The data was published in the journal Proteome Sci in 2013. PMID: 23866759

REFERENCE

REVIEW

Peroxiredoxin 6 antibody Cat. No. GTX115262

Rating ( Average 5 based on 10 users reviews)
Application
Western Blot(WB) ( Average 5 based on 8 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 23-Jan-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : HEK293 cells
Primary Antibody : 1:1000, 4°C, >12Hr
Date : Anonymous submitted on 17-Nov-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : Mouse brain tissue lysate
Blocking : Block Ace, 24°C, 0.5Hr
Primary Antibody : 1:3000, 15°C, 16Hr
Date : Anonymous submitted on 22-Jun-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HAP1 WT and PRDX6 KO cells
Blocking : 5% BSA, 5% goat serum, and 0.01% Triton X-100 in PBS, 30 min, RT
Fixation : 4% PFA in PBS, 15 min, RT
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in PBS, 10 min, RT
Primary Antibody : 1:1000 in 5% BSA, 5% goat serum, and 0.01% Triton X-100 in PBS, 4°C, > 12Hr
Lane Description :
Yellow dashed line : WT cells
Magenta dashed line : KO cells
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date : Anonymous submitted on 22-Jun-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description :
SM : 10% starting material
UB : 10% unbound fraction
IP : IP antibody
Figure Note : 2.0μg of antibody to 500μl of PBS with 0.01% triton X-100, together with 30μl of protein A-Sepharose beads. One ml aliquots at 0.5 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date : Anonymous submitted on 22-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and PRDX6 KO cells
Amount used : 25μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:1000 5% BSA in TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : milkfish
Figure Note : The data was peer reviewed and published in the journal Front Physiol in 2016. PMID: 27965586
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : milkfish
Figure Note : The data was peer reviewed and published in the journal Front Physiol in 2016. PMID: 27965586
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : milkfish
Figure Note : The data was peer reviewed and published in the journal Front Physiol in 2016. PMID: 27965586
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal J Biomed Sci in 2013. PMID: 24359494
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Proteome Sci in 2013. PMID: 23866759
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169