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Peroxiredoxin 6 antibody

KO/KD
Anti-Peroxiredoxin 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32779
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX32779

Cat. No. GTX32779

3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Peroxiredoxin 6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IP Assay dependent
Not tested in other applications.

Calculated MW

25 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-224 of human PRDX6 (NP_004896.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxiredoxin 6 , 1-Cys , AOP2 , HEL-S-128m , NSGPx , PRX , aiPLA2 , p29

Cellular Localization

Cytoplasm,Lysosome

Background

The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Peroxiredoxin 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32779

GTX32779 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32779 Peroxiredoxin 6 antibody.

Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX32779

GTX32779 WB Image

WB analysis of various sample lysates using GTX32779 Peroxiredoxin 6 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

There are currently no references for Peroxiredoxin 6 antibody (GTX32779). Be the first to share your publications with this product.
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REVIEW

Peroxiredoxin 6 antibody Cat. No. GTX32779

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-Peroxiredoxin 6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32779
Date : Anonymous submitted on 22-Jun-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HAP1 WT and PRDX6 KO cells
Blocking : 5% BSA, 5% goat serum, and 0.01% Triton X-100 in PBS, 30 min, RT
Fixation : 4% PFA in PBS, 15 min, RT
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in PBS, 10 min, RT
Primary Antibody : 1:1000 in 5% BSA, 5% goat serum, and 0.01% Triton X-100 in PBS, 4°C, > 12Hr
Lane Description :
Yellow dashed line : WT cells
Magenta dashed line : KO cells
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Peroxiredoxin 6 antibody used in Immunoprecipitation (IP). GTX32779
Date : Anonymous submitted on 22-Jun-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description :
SM : 10% starting material
UB : 10% unbound fraction
IP : IP antibody
Figure Note : 2.0μg of antibody to 500μl of PBS with 0.01% triton X-100, together with 30μl of protein A-Sepharose beads. One ml aliquots at 0.5 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-Peroxiredoxin 6 antibody used in Western Blot (WB). GTX32779
Date : Anonymous submitted on 22-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and PRDX6 KO cells
Amount used : 25μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:5000 in 5% BSA in TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399