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Pescadillo antibody

Anti-Pescadillo antibody used in Western Blot (WB). GTX47602
Anti-Pescadillo antibody used in IHC (Paraffin sections) (IHC-P). GTX47602

Cat. No. GTX47602

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μg ($399)
See all Pescadillo products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-3μg/ml
IHC-P 2.5-3.8μg/ml

Note :

IHC-P
Human Testis shows nucleolar staining in spermatocytes and spermatids.
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX47602-PEP).

Predict Reactivity

Mouse, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence GGLEKKKYERGSA-C, from the N Terminus of the protein sequence according to NP_055118.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

pescadillo ribosomal biogenesis factor 1 , NOP7 , PES

Cellular Localization

nucleoplasm , Nucleolus

Background

This gene encodes a nuclear protein that contains a breast cancer associated gene 1 (BRCA1) C-terminal interaction domain. The encoded protein interacts with BOP1 and WDR12 to form the PeBoW complex, which plays a critical role in cell proliferation via pre-rRNA processing and 60S ribosomal subunit maturation. Expression of this gene may play an important role in breast cancer proliferation and tumorigenicity. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the long arm of chromosome 4 and the short arm of chromosome 9. [provided by RefSeq, Aug 2011]

Database

Research Area

DATA IMAGES

Anti-Pescadillo antibody used in Western Blot (WB). GTX47602

GTX47602 WB Image

WB analysis of HeLa lysate using GTX47602 Pescadillo antibody.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

Anti-Pescadillo antibody used in IHC (Paraffin sections) (IHC-P). GTX47602

GTX47602 IHC-P Image

IHC-P analysis of human testis using GTX47602 Pescadillo antibody.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.8μg/ml

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399