GeneTex
  • Country / Location Selection

United States (US)

Phalloidin (TRITC)

Cat No. GTX66876

Application ICC/IF
Species Amanita phalloides
Package
100 μg ($369)
PRODUCT

Product Description

Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. Fluorescent conjugates of phalloidin are used to label actin filaments for histological applications. Some structural features of phalloidin are required for the binding to actin.
APPLICATION

Application Note

Stock solutions of phalloidin conjugates have been made in methanol or DMSO at 0.1–5 mg/ml. Final staining solutions in aqueous physiological buffers are in the concentration range of 1 - 50 μg/ml with corresponding incubation times of 15 minutes to 72 hours. The following procedure may be used as a guideline for staining cells:
1. Cells are washed with phosphate buffered saline (PBS).
2. Cells are fixed for 5 minutes in 3.7% formaldehyde solution in PBS. Then washed extensively in PBS.
3. Cells are permeabilized with 0.1% Triton X-100 in PBS, and washed again in PBS.
4. Cells are stained with a fluorescent phalloidin conjugate solution in PBS (containing 1% DMSO from the original stock solution) for 40 minutes at room temperature.
5. Wash several times with PBS to remove unbound phalloidin conjugate.
PROPERTIES

Form

Lyophilized powder

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4oC. For long-term storage, aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. Protect from light.

Conjugation

Rhodamine
Rhodamine (TRITC)

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
REFERENCE
There are currently no references for Phalloidin (TRITC) (GTX66876). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Phalloidin (TRITC) (GTX66876). Be the first to share your experience with this product.
Package List Price ($)
$ 369