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Phb antibody

Anti-Phb antibody used in IHC (Whole mount) (IHC-Wm). GTX124491
Anti-Phb antibody used in Western Blot (WB). GTX124491

Cat. No. GTX124491

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Wm

Reactivity

 Zebrafish, Teleost
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Phb products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-Wm 1:100-1:500
Not tested in other applications.

Predict Reactivity

Human (100%)| Mouse (99%)| Rat (99%)| Zebrafish (93%)| Bovine (100%)| Cat (100%)| Dog (100%)| Chicken (95%)| Xenopus tropicalis (93%)| Chimpanzee (100%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.89 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Phb

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11169327

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

prohibitin , fa18f07 , fb09f05 , sr:nyz128 , wu:fa18f07 , wu:fb09f05

Database

Research Area

DATA IMAGES

Anti-Phb antibody used in IHC (Whole mount) (IHC-Wm). GTX124491

GTX124491 IHC-Wm Image

Phb antibody detects Phb protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Phb antibody (GTX124491) dilution: 1:100.

Anti-Phb antibody used in Western Blot (WB). GTX124491

GTX124491 WB Image

Various tissue extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Phb antibody (GTX124491) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Xiong Z et al. Asian-Australas J Anim Sci 2018; 31 (11) : 1729-1737 Expression pattern of prohibitin, capping actin protein of muscle Z-line beta subunit and tektin-2 gene in Murrah buffalo sperm and its relationship with sperm motility.
Chauvigne F et al. Sci Rep 2015; 5 : 7789 Mitochondrial aquaporin-8-mediated hydrogen peroxide transport is essential for teleost spermatozoon motility.
Application : WB
Reactivity : Teleost
Boj M et al. Biol Reprod 2015; 93 (2) : 40 Coordinated Action of Aquaporins Regulates Sperm Motility in a Marine Teleost.
Application : WB
Reactivity : Teleost
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169