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Phosducin / PDC antibody

Anti-Phosducin / PDC antibody used in IHC (Paraffin sections) (IHC-P). GTX131327
Anti-Phosducin / PDC antibody used in Western Blot (WB). GTX131327

Cat. No. GTX131327

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse
Package
100 μl ($399),
25 μl ($169)
See all Phosducin / PDC products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

mouse eye

Predict Reactivity

Human, Rat, Bovine, Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.06 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Phosducin / PDC. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886461

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

phosducin , MEKA , PHD , PhLOP , PhLP

Background

This gene encodes a phosphoprotein, which is located in the outer and inner segments of the rod cells in the retina. This protein may participate in the regulation of visual phototransduction or in the integration of photoreceptor metabolism. It modulates the phototransduction cascade by interacting with the beta and gamma subunits of the retinal G-protein transducin. This gene is a potential candidate gene for retinitis pigmentosa and Usher syndrome type II. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Phosducin / PDC antibody used in IHC (Paraffin sections) (IHC-P). GTX131327

GTX131327 IHC-P Image

Phosducin / PDC antibody detects PDC protein at cytoplasm in mouse eye by immunohistochemical analysis.
Sample: Paraffin-embedded mouse eye.
Phosducin / PDC antibody (GTX131327) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Phosducin / PDC antibody used in Western Blot (WB). GTX131327

GTX131327 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Phosducin / PDC antibody (GTX131327) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169