GeneTex
United States (US)

Phospholamban antibody, N-term

GTX88052_1068_IHC-P_18122102_529.jpg
GTX88052_2566_WB_18122102_142.jpg

Cat No. GTX88052

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.01-0.03μg/ml
IHC-P 3.75μg/ml
Not tested in other applications.

Calculated MW

6 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88052-PEP).

Predict Reactivity

Bovine, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence KVQYLTRSAIRR-C, from the N Terminus of the protein sequence according to NP_002658.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phospholamban,Cmd1P,Cmh18,Plb,Pln

Background

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX88052_1068_IHC-P_18122102_529.jpg

GTX88052 IHC-P Image

IHC-P analysis of human heart using GTX88052 Phospholamban antibody, N-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 3.75µg/ml

GTX88052_2566_WB_18122102_142.jpg

GTX88052 WB Image

WB analysis of human heart lysate using GTX88052 Phospholamban antibody, N-term.
Dilution : 0.03µg/ml
Loading : 35µg protein in RIPA buffer

Package List Price ($)
$ 329