GeneTex

  • Country / Location Selection

United States (US)

Phospholamban (phospho Ser16) antibody

Orthogonal
Anti-Phospholamban (phospho Ser16) antibody used in Western Blot (WB). GTX15000

Cat. No. GTX15000

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human, Rabbit, Bovine
Package
50 μg ($449)
See all Phospholamban products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.5-2μg/ml (bovine cardiac preparation incubated with 10μg PKA for 10 minutes)

Note :

WB
Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.
Not tested in other applications.

Calculated MW

6 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine pH7.4, 0.15M Sodium Chloride, 30% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to aa14-25, RA[pS]TIEMPQQAR-C of human cardiac phospholamban with a cysteine added at the C-terminal, conjugated to KLH.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_368930

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

CMD1P , CMH18 , PLB , PLN , phospholamban , Phospholamban

Cellular Localization

Endoplasmic reticulum membrane,Sarcoplasmic reticulum membrane,Mitochondrion membrane,Membrane

Background

The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy. [provided by RefSeq, Apr 2016]

Database

Research Area

DATA IMAGES

Anti-Phospholamban (phospho Ser16) antibody used in Western Blot (WB). GTX15000

GTX15000 WB Image

Orthogonal

WB analysis of bovine cardiac preparation untreated or treated with PKA using GTX15000 Phospholamban (phospho Ser16) antibody.
Dilution : 2μg/ml

REFERENCE

Application Reference

Huang SY et al. J Cell Mol Med 2021; 25 (24) : 11264-11277 Calcium dysregulation increases right ventricular outflow tract arrhythmogenesis in rabbit model of chronic kidney disease.
Application : WB
Reactivity : Rabbit
Chang PC et al. J Card Fail 2020; Sacubitril/Valsartan Therapy Ameliorates Ventricular Tachyarrhythmia Inducibility in a Rabbit Myocardial Infarction Model.
Application : WB
Reactivity : Rabbit
Sroubek J et al. J Biol Chem 2011; 286 (24) : 21927-36 Protein kinase A activity at the endoplasmic reticulum surface is responsible for augmentation of human ether-a-go-go-related gene product (HERG).
Submit a Reference

REVIEW

There are currently no reviews for Phospholamban (phospho Ser16) antibody (GTX15000). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 449