GeneTex
United States (US)

Phospholamban (phospho Ser16) antibody

Cat No. GTX15000

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human, Mouse, Rat, Rabbit, Bovine, Dog, Pig
APPLICATION

Application Note

WB: Use at a concentration of 0.5 - 2 μg/ml. Predicted molecular weight: 5-25 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

6 kDa. ( Note )

Positive Control

Bovine cardiac preparation
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.05% Sodium Azide; Constituents: 30% Glycerol, 0.2M Tris glycine, 0.15M Sodium chloride

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated, synthetic peptide corresponding to amino acids 14-25 of human cardiac phospholamban (RA[pS]TIEMPQQAR-C) with an added C-terminal cysteine. The immunizing sequence is identical in mouse, rat, rabbit, pig and dog.

Purification

Immunogen affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Phospholamban,Cmd1P,Cmh18,Plb,Pln

Cellular Localization

Membrane

Background

Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.

Database

Research Area

REFERENCE
Package List Price ($)
$ 339