GeneTex
United States (US)

Phosphoserine antibody (Biotin)

Phosphoserine-antibody-Biotin-GTX29335-WB-1_18121411_822.jpg
Phosphoserine-antibody-Biotin-GTX29335-WB-3_18121411_765.jpg
Phosphoserine-antibody-Biotin-GTX29335-WB-2_18121411_545.jpg

Cat No. GTX29335

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, ELISA
APPLICATION

Application Note

For ELISA: Use at a concentration of 0.5 μg/mL. For WB: Use at a concentration of 4μg/mL. For IP: Use at a concentration of 10 μg/250 μg chromatography fractionated protein sample. Detects 50 ng of phosvitin with immunoblotting and 0.5 ng of phosvitin with immunocaptured ELISA. Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.

Specificity/Sensitivity

Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.
PROPERTIES

Form

Liquid

Buffer

Phosphate-buffered saline containing 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.25mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

BSA and KLH-phosphoserine conjugates.

Purification

Immunogen affinity purified
Immunoaffinity chromatography with phosphoserine-agarose.

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DATA IMAGES
Phosphoserine-antibody-Biotin-GTX29335-WB-1_18121411_822.jpg

GTX29335 WB Image

The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E).

Phosphoserine-antibody-Biotin-GTX29335-WB-3_18121411_765.jpg

GTX29335 WB Image

Immunoblotting of BSA-S6 peptide phosphorylated by partial purified S6K with anti-phosphoserine. S6 peptide (CKRRRLASLR) coupled to BSA was phosphorylated with calf-brain S6K for 2, 10, 30, and 60 min, lane A,B,C,D, respectively.

Phosphoserine-antibody-Biotin-GTX29335-WB-2_18121411_545.jpg

GTX29335 WB Image

Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

Package List Price ($)
$ 329