GeneTex

  • Country / Location Selection

United States (US)

Phosphoserine antibody (Biotin)

Anti-Phosphoserine antibody (Biotin) used in Western Blot (WB). GTX29335
Anti-Phosphoserine antibody (Biotin) used in Western Blot (WB). GTX29335
Anti-Phosphoserine antibody (Biotin) used in Western Blot (WB). GTX29335

Cat. No. GTX29335

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Species independent
Package
50 μg ($399)
See all Phosphoserine products

APPLICATION

Application Note

For ELISA: Use at a concentration of 0.5 μg/mL. For WB: Use at a concentration of 4μg/mL. For IP: Use at a concentration of 10 μg/250 μg chromatography fractionated protein sample. Detects 50 ng of phosvitin with immunoblotting and 0.5 ng of phosvitin with immunocaptured ELISA. Do not use milk as a blocking agent or in diluents, as milk casein is phosphorylated at several serine residues. BSA is recommended instead. Optimal dilutions/concentrations should be determined by the researcher.

Positive Control

Use Mouse brain extract for Western Blotting or synthetic phosphopeptide or phosvitin for ELISA.

Product Note

Specifically recognizes both free-phosphoserine, serine-phosphorylated peptide and proteins. Does not cross-react with ATP, phosphotyrosine, peptidyl phosphothreonine and serine. Slight cross-reactivity with free phosphothreonine. Readily reacts to known phosphoproteins such as phosvitin and alpha casein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

BSA and KLH-phosphoserine conjugates.

Purification

Immunogen affinity purified
Immunoaffinity chromatography with phosphoserine-agarose.

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Research Area

DATA IMAGES

Anti-Phosphoserine antibody (Biotin) used in Western Blot (WB). GTX29335

GTX29335 WB Image

The detected band is 53 kD, and is a similar size to the p53 tumor suppressor factor. The cells were treated with 0, 50, 200 or 400 J UV (lane A to D, respectively) and with 0.1uM of okadaic acid(lane E).

Anti-Phosphoserine antibody (Biotin) used in Western Blot (WB). GTX29335

GTX29335 WB Image

Immunoblotting of BSA-S6 peptide phosphorylated by partial purified S6K with anti-phosphoserine. S6 peptide (CKRRRLASLR) coupled to BSA was phosphorylated with calf-brain S6K for 2, 10, 30, and 60 min, lane A,B,C,D, respectively.

Anti-Phosphoserine antibody (Biotin) used in Western Blot (WB). GTX29335

GTX29335 WB Image

Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

REFERENCE

There are currently no references for Phosphoserine antibody (Biotin) (GTX29335). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Phosphoserine antibody (Biotin) (GTX29335). Be the first to share your experience with this product.
Submit a Review
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399