WB analysis of EGF-treated A431 whole cell lysate using GTX22286 Phosphothreonine antibody [18F6].
Dilution : 1:1000

ELISA analysis of BSA-conjugated pT, pY and pS using serially diluted GTX22286 Phosphothreonine antibody [18F6].
Coating : 0.1 μg
Dilution : 1:1000

ELISA results of Mab anti-phosphothreonine antibody (GTX22286) tested against BSA conjugates of pT, pY and pS. Each well was coated with 0.1μg of conjugate. The starting dilution of antibody was 1:1000 and each point on the X-axis represents a 2-fold dilution. HRP conjugated Gt-a-Mouse IgG (H&L) antibody and TMB substrate were used for detection.

Mab anti-phosphothreonine antibody (clone 18F6, GTX22286) is shown to detect threonine phosphorylation of proteins in a lysate from A431 cells stimulated with EGF. Separation is achieved under reducing conditions using a pre-cast 4-20% iGel from Gradipore, Inc. A 1:1,000 dilution of Mab anti-phosphothreonine is used for 30 min followed by detection using 1:2,500 dilution Goat-a-Mouse IgG [H&L] secondary antibody and visualization using the OdysseyR Infrared Imaging System developed by LI-COR.