IP analysis of Calyculin A/Okadaic Acid treated A431 cells using GTX60860 Phosphothreonine antibody [RM102].
Lane 1 : Whole lysate control
Lane 2 : IP by rabbit IgG control
Lane 3 : IP by Primary antibody
Dilution : 1:500

ICC/IF analysis of A431 cells nontreated or treated with Calyculin A/Okadaic Acid using GTX60860 Phosphothreonine antibody [RM102].
Red : Primary antibody
Blue : DAPI
Dilution : 1:500

WB analysis of serum-starved A431 cells non-treated or treated with Calyculin A/Okadaic Acid lysates using GTX60860 Phosphothreonine antibody [RM102].
Dilution : 1:2000

GTX60860 recognizes Phosphorylated threonine in peptides with different sequences. It has minimal cross-reactivity with phosphorylated serine.