ELISA(kinase assay): Use at 0.5 μg/mLWestern blot: Use at 4μg/mLIP: Use at 10 μg/250 μg protein sampleWill detect 100 ng of phosvitin in Western Blots and 0.5 ng of phosvitin with ELISA.Can be used for non-radioactive protein kinase assay (ELISA) using biotinylated peptide substrate and immunoblotting of abundant phosphoproteins. It is not recommended for immunoblotting of trace cellular phosphoproteins. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation.
Use mouse brain extract for immunoblotting. Use synthetic phosphopeptide (phosphorylated on threonine) for ELISA.
Reacts with free phosphothreonine but does not react with phosphoserine, threonine or phosphotyrosine.
TBS with 0.01% sodium azide
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.
1mg/ml(Please refer to the vial label for the specific concentration.)
Chemical / Small Molecule conjugated to KLH.
Immunogen affinity purified
Immunoaffinity chromatography with phosphothreonine-agarose.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.