GeneTex
United States (US)

Phosphothreonine antibody

Phosphothreonine-antibody-GTX29337-ELISA-1_18121411_699.jpg
Phosphothreonine-antibody-GTX29337-WB-1_18121411_479.jpg
Phosphothreonine-antibody_WB_GTX29337-1_18121411_322.jpg

Cat No. GTX29337

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP, ELISA
APPLICATION

Application Note

ELISA(kinase assay): Use at 0.5 μg/mLWestern blot: Use at 4μg/mLIP: Use at 10 μg/250 μg protein sampleWill detect 100 ng of phosvitin in Western Blots and 0.5 ng of phosvitin with ELISA.Can be used for non-radioactive protein kinase assay (ELISA) using biotinylated peptide substrate and immunoblotting of abundant phosphoproteins. It is not recommended for immunoblotting of trace cellular phosphoproteins. Acetone precipitation of the protein extract followed by SDS denaturation is recommended for successful immunoprecipitation.

Positive Control

Use mouse brain extract for immunoblotting. Use synthetic phosphopeptide (phosphorylated on threonine) for ELISA.

Specificity/Sensitivity

Reacts with free phosphothreonine but does not react with phosphoserine, threonine or phosphotyrosine.
PROPERTIES

Form

Liquid

Buffer

TBS with 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

Chemical / Small Molecule conjugated to KLH.

Purification

Immunogen affinity purified
Immunoaffinity chromatography with phosphothreonine-agarose.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Phosphorylation of threonine residues is associated with many growth factors and oncogene protein kinases, and is important for cell signaling in activation, proliferation and differentiation.
DATA IMAGES
Phosphothreonine-antibody-GTX29337-ELISA-1_18121411_699.jpg

GTX29337 ELISA Image

Antibody Capture ELISA. Label: immobilized antigen

Phosphothreonine-antibody-GTX29337-WB-1_18121411_479.jpg

GTX29337 WB Image

Immunoblotting of fetal mouse brain extract (125 ug - A and 25 ug - B)

Phosphothreonine-antibody_WB_GTX29337-1_18121411_322.jpg

GTX29337 WB Image

Western blot analysis of mouse spleen extract probed with GTX29337.
Left Lane: Vanadium treated
Right lane: Untreated

Package List Price ($)
$ 369