WB analysis of various samples using GTX22287 Phosphotyrosine antibody [13F9].
Lane 1 : Phosphorylated EGFR in A431 whole cell lysate
Lane 2 : Unstimulated A431 whole cell lysate
Loading : 10 μg
Dilution : 1:1000

ELISA analysis of BSA-conjugated pT, pY and pS using serially diluted GTX22287 Phosphotyrosine antibody [13F9].
Coating : 0.1 μg
Dilution : 1:1000

ELISA of Mouse anti-phosphotyrosine antibody (GTX22287). Antigen: BSA conjugates of pT, pY and pS. Coating amount: 0.1 μg per well. Primary antibody: Phosphotyrosine antibody at 1:1000. Dilution series (X-axis): 2-fold. Secondary antibody: HRP conjugated Gt-a-Mouse IgG H&L secondary antibody at 1:20,000. Substrate: TMB

Western Blot of Mouse anti-phosphotyrosine antibody (clone 13F9) (GTX22287). Lane 1: phosphorylated EGFR in an A431 cell lysate. Lane 2: total protein in the unstimulated A431 cells lysate after coommassie staining. Load: 10 μg per lane. Primary antibody: phosphotyrosine antibody at 1:1000 for 1 hr at RT. Secondary antibody:Goat-a-Mouse IgG [H&L] secondary antibody diluted 1:2,500 for 30' at room temperature. Block: 5% BLOTTO overnight at 4ºC.