GeneTex
United States (US)

Phosphotyrosine antibody [P-Tyr-01]

Phosphotyrosine-antibody-P-TYR-01__GTX28076-1_18121411_595.jpg

Cat No. GTX28076

Host Mouse
Clonality Monoclonal
Clone Name P-Tyr-01
Isotype IgG1
Application WB, ICC/IF
APPLICATION

Application Note

ICC: Use at an assay dependant dilution. WB: Use at an assay dependant concentration. Optimal dilutions/concentrations should be determined by the end user.

Positive Control

Jurkat cells stimulated with anti CD3 antibody (2 min incubation)

Specificity/Sensitivity

Phosphotyrosine.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline pH 7.4 containing 15mM sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Immunogen

Phosphotyrosine conjugated to BSA.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

Detection of activation-induced tyrosine phosphorylation by Western Blotting.
DATA IMAGES
Phosphotyrosine-antibody-P-TYR-01__GTX28076-1_18121411_595.jpg

GTX28076 ICC/IF Image

Phosphotyrosine pattern in HeLa cells as revealed by P-Tyr-01 antibody (GTX28076) -Alexa Fluor® 488 (green). Actin detected by phalloidin-Alexa Fluor® 567, DNA stained by DAPI (blue).

Package List Price ($)
$ 319