GeneTex
United States (US)

Phosphotyrosine antibody [PY20] (Biotin)

Cat No. GTX16388

Host Mouse
Clonality Monoclonal
Clone Name PY20
Isotype IgG2b
Application WB, ICC/IF, ELISA, IHC
APPLICATION

Application Note

ELISA: 1/5000. ICC: 1/500. WB: 1/5000. IHC: 1/500. Optimal dilutions/concentrations should be determined by the end user.

Specificity/Sensitivity

Clone PY20 specifically recognizes phosphorylated tyrosine residues and does not react with phosphorylated threonine or serine residues.
PROPERTIES

Form

Liquid

Buffer

Preservative: 3mM Sodium Azide. Constituents: 50% Glycerol, 0.15M Sodium chloride, 0.02M Sodium phosphate. pH 7.5

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

Phosphotyrosine coupled to carrier protein

Purification

Immunogen affinity purified

Conjugation

Biotin

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Background

The role of tyrosine phosphorylation in transduction of the mitogenic signal from transmembrane receptors and in transformation by oncogene tyrosine kinases has been the subject of intense investigation for several years. While the phosphorylation of specific tyrosine residues has been shown to be a primary mechanism of signal transduction during normal mitogenesis, cell cycle progression and oncogenic transformation, its role in other areas such as differentiation and gap junction communication, is a matter of active and ongoing research.
Package List Price ($)
$ 319