GeneTex

  • Country / Location Selection

United States (US)

PiT2 antibody [N2C1], Internal

Anti-PiT2 antibody [N2C1], Internal used in Western Blot (WB). GTX105078
Anti-PiT2 antibody [N2C1], Internal used in Western Blot (WB). GTX105078

Cat. No. GTX105078

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all PiT2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000

Note :

WB
As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

A431 , Unboiled U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Cat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.46 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PiT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951923

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 20 member 2 , GLVR-2 , GLVR2 , IBGC1 , IBGC3 , MLVAR , PIT-2 , PIT2 , RAM1 , Ram-1

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Sodium-phosphate symporter which seems to play a fundamental housekeeping role in phosphate transport by absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. In vitro, sodium-dependent phosphate uptake is not siginificantly affected by acidic and alkaline conditions, however sodium-independent phosphate uptake occurs at acidic conditions. May play a role in extracellular matrix, cartilage and vascular calcification. Functions as a retroviral receptor and confers human cells susceptibility to infection to amphotropic murine leukemia virus (A-MuLV), 10A1 murine leukemia virus (10A1 MLV) and some feline leukemia virus subgroup B (FeLV-B) variants.

Database

Research Area

DATA IMAGES

Anti-PiT2 antibody [N2C1], Internal used in Western Blot (WB). GTX105078

GTX105078 WB Image

A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PiT2 antibody [N2C1], Internal (GTX105078) diluted at 1:500.

Anti-PiT2 antibody [N2C1], Internal used in Western Blot (WB). GTX105078

GTX105078 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PiT2 antibody [N2C1], Internal (GTX105078) diluted at 1:500.

REFERENCE

There are currently no references for PiT2 antibody [N2C1], Internal (GTX105078). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for PiT2 antibody [N2C1], Internal (GTX105078). Be the first to share your experience with this product.
Submit a Review
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169