GeneTex
United States (US)

PiT2 antibody [N2C1], Internal

GTX105078_39904_20160414_WB_19011506_770.jpg
GTX105078_39904_20160414_WB_2_19011506_761.jpg

Cat No. GTX105078

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000

Note :

As is commonly seen with membrane proteins, significant hydrophobicity can lead to aggregation following boiling of samples prior to SDS-PAGE and subsequent western blotting. We recommend to avoid boiling in this case.
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Unboiled U87-MG , Boiled A431 , Unboiled A431

Predict Reactivity

Mouse, Rat, Bovine, Cat, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.46mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human PiT2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

GLVR2 antibody, Glvr-2 antibody, MLVAR antibody, PIT-2 antibody, SLC20A2 antibody, hPit2 antibody, phosphate transporter 2 antibody, sodium-dependent phosphate transporter 2 antibody, solute carrier family 20 member 2 antibody, "murine leukemia virus, amphotropic, receptor for antibody", gibbon ape leukemia virus receptor 2 antibody, pit2 antibody, "solute carrier family 20 (phosphate transporter), member 2 antibody"

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

Sodium-phosphate symporter which seems to play a fundamental housekeeping role in phosphate transport by absorbing phosphate from interstitial fluid for normal cellular functions such as cellular metabolism, signal transduction, and nucleic acid and lipid synthesis. In vitro, sodium-dependent phosphate uptake is not siginificantly affected by acidic and alkaline conditions, however sodium-independent phosphate uptake occurs at acidic conditions. May play a role in extracellular matrix, cartilage and vascular calcification. Functions as a retroviral receptor and confers human cells susceptibility to infection to amphotropic murine leukemia virus (A-MuLV), 10A1 murine leukemia virus (10A1 MLV) and some feline leukemia virus subgroup B (FeLV-B) variants.
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Database

DATA IMAGES
GTX105078_39904_20160414_WB_19011506_770.jpg

GTX105078 WB Image

A431 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PiT2 antibody [N2C1], Internal (GTX105078) diluted at 1:500.

GTX105078_39904_20160414_WB_2_19011506_761.jpg

GTX105078 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PiT2 antibody [N2C1], Internal (GTX105078) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169