GeneTex

  • Country / Location Selection

United States (US)

Pig IL6 protein, His tag (active)

Pig IL6 protein, His tag (active). GTX00338-pro
Pig IL6 protein, His tag (active). GTX00338-pro
Pig IL6 protein, His tag (active). GTX00338-pro

Cat. No. GTX00338-pro

Application

 Functional Assay

Species

 Pig
Package
10 μg ($299)
See all IL6 protein products

APPLICATION

Application Note

Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with 1% serum standard RPMI-1640 which contains various concentrations of recombinant pig IL6. After incubated for 5 days,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of Raji cells after incubation with IL6 for 5 days observed by inverted microscope.

Observed MW

26 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 20mM Tris and 150mM NaCl to 0.1-1.0mg/ml. Do not vortex. Lyophilized from 20mM Tris, 150mM NaCl, 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro29~Met212 (NP_001239358.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

interleukin 6

Database

Research Area

DATA IMAGES

Pig IL6 protein, His tag (active). GTX00338-pro

GTX00338-pro Functional Assay Image

Cell proliferation effect of GTX00338-pro Pig IL6 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated Raji cells cultured in RPMI-1640 for 5 days.
(B) Raji cells cultured in RPMI-1640, stimulated with 10 ng/ml IL6 for 5 days.

Pig IL6 protein, His tag (active). GTX00338-pro

GTX00338-pro Image

SDS-PAGE analysis of GTX00338-pro Pig IL6 protein (active).

Pig IL6 protein, His tag (active). GTX00338-pro

GTX00338-pro Image

WB analysis of GTX00338-pro Pig IL6 protein (active).

REFERENCE

There are currently no references for Pig IL6 protein, His tag (active) (GTX00338-pro). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for Pig IL6 protein, His tag (active) (GTX00338-pro). Be the first to share your experience with this product.
Submit a Review
SDS
Proclin.pdf
Package List Price ($)
$ 299