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Pig IL6 protein (active)

Pig IL6 protein (active). GTX00338-pro
Pig IL6 protein (active). GTX00338-pro
Pig IL6 protein (active). GTX00338-pro

Cat No. GTX00338-pro

Application

Functional Assay

Species

Pig
Package
10 μg ($299)

APPLICATION

Application Note

Interleukin 6 (IL-6) is an interleukin that acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Interleukin 6 is secreted by T cells and macrophages to stimulate immune response and also plays a role in fighting infection. It supports the growth of B cells and is antagonistic to regulatory T cells. To test the effect of IL6 on cell proliferation, Raji cells were seeded into triplicate wells of 96-well plates at a density of 5000 cells/well with 1% serum standard RPMI-1640 which contains various concentrations of recombinant pig IL6. After incubated for 5 days,  cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 μl of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37ºC. Proliferation of Raji cells after incubation with IL6 for 5 days observed by inverted microscope.

Observed MW

26 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Lyophilized from 20 mM Tris (pH 8.0) with 150 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.01% SKL, 5% Trehalose, Proclin300. Reconstitute with 20 mM Tris and 150 mM NaCl (pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution.Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Pro29~Met212 (NP_001239358.1)

Expression System

E. coli

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Pig IL6 protein (active). GTX00338-pro

GTX00338-pro Functional Assay Image

Cell proliferation effect of GTX00338-pro Pig IL6 protein (active). Cell viability was measured by Cell Counting Kit-8 (CCK-8).
(A) Unstimulated Raji cells cultured in RPMI-1640 for 5 days.
(B) Raji cells cultured in RPMI-1640, stimulated with 10 ng/ml IL6 for 5 days.

Pig IL6 protein (active). GTX00338-pro

GTX00338-pro Image

WB analysis of GTX00338-pro Pig IL6 protein (active).

Pig IL6 protein (active). GTX00338-pro

GTX00338-pro Image

SDS-PAGE analysis of GTX00338-pro Pig IL6 protein (active).

REFERENCE

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REVIEW

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