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Pig SCF protein, His tag (active)

Pig SCF protein, His tag (active). GTX00337-pro
Pig SCF protein, His tag (active). GTX00337-pro
Pig SCF protein, His tag (active). GTX00337-pro
Pig SCF protein, His tag (active). GTX00337-pro

Cat. No. GTX00337-pro

Application

 Functional Assay

Species

 Pig
Package
10 μg ($349)
See all SCF protein products

APPLICATION

Application Note

Stem cell factor (SCF), also known as mast cell growth factor (MGF), and steel factor (SLF), plays an important role in hematopoiesis, spermatogenesis, and melanogenesis. SCF has been shown to stimulate the proliferation of TF-1 cells. To test this effect,  TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 1x1x10⁴cells/well and incubated for 72h in the presence or absence of various concentrations of SCF at 37ºC.   Cell proliferation of TF-1 cells after incubation with SCF FOR 72h was observed by inverted microscope.

Observed MW

20 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with 10mM PBS (pH7.4) to 0.1-1.0mg/ml. Do not vortex. Lyophilized from PBS (pH7.4), 0.01% SKL, 1mM DTT, 5% Trehalose.

Preservative

ProClin 300

Storage

For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, store at -20ºC or below. After reconstitution, keep as concentrated solution. Avoid freeze-thaw cycles.

Region/Sequence

N-terminal His-Tag; Thr25~Ala191 (NP_999434.2)

Expression System

HEK293 cells

Purity

> 95%

Endotoxin

< 1 EU/μg

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

KIT ligand , KITL

Database

Research Area

DATA IMAGES

Pig SCF protein, His tag (active). GTX00337-pro

GTX00337-pro Functional Assay Image

The dose effect curve of GTX00337-pro Pig SCF protein (active) on TF-1 cells. After incubation of Pig SCF protein (active) for 72 hrs, cell proliferation was measured by Cell Counting Kit-8 (CCK-8).

Pig SCF protein, His tag (active). GTX00337-pro

GTX00337-pro Functional Assay Image

Cell proliferation effect of GTX00337-pro Pig SCF protein (active).
(A) Unstimulated TF-1 cells cultured in RPMI-1640 for 72hrs.
(B) TF-1 cells cultured in RPMI-1640, stimulated with 10 ng/ml SCF for 72hrs.

Pig SCF protein, His tag (active). GTX00337-pro

GTX00337-pro Image

SDS-PAGE analysis of GTX00337-pro Pig SCF protein (active).

Pig SCF protein, His tag (active). GTX00337-pro

GTX00337-pro Image

WB analysis of GTX00337-pro Pig SCF protein (active).

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SDS
PBS.pdf
Proclin.pdf
Package List Price ($)
$ 349