Pirh2 antibody [1H10]

Cat. No. GTX83290

Host	Mouse
Clonality	Monoclonal
Clone Name	1H10
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS, ELISA
Reactivity	Human, Rat
Package	100 μl ($399)

See all Pirh2 products

Datasheet
Document

View product citations for antibody GTX83290 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1/500 - 1/2000
ICC/IF	1/200 - 1/1000
IHC-P	1/200 - 1/1000
FACS	1/200 - 1/400
ELISA	1/10000

Not tested in other applications.

Calculated MW

30 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human Pirh2 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger and CHY zinc finger domain containing 1 , ARNIP , CHIMP , PIRH2 , PRO1996 , RNF199 , ZCHY , ZNF363

Cellular Localization

Nucleus,Nucleus speckle,Cytoplasm

Background

The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including TP53, HDAC1 and CDKN1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Feb 2011]

Database

Gene ID: 25898 RCHY1
UniProt: Q96PM5 RCHY1

Research Area

DATA IMAGES

GTX83290 WB Image

WB analysis of HeLa (1), A549 (2), MCF-7 (3) and PC-12 (4) cell lysate using GTX83290 Pirh2 antibody [1H10].

GTX83290 IHC-P Image

IHC-P analysis of human Tonsil tissue using GTX83290 Pirh2 antibody [1H10].

GTX83290 FACS Image

FACS analysis of PC-12 cells using GTX83290 Pirh2 antibody [1H10].
Blue : Pirh2
Red : negative control

GTX83290 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX83290 Pirh2 antibody [1H10].
Green : Pirh2
Red: Actin filaments

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 399

Qty