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Pirh2 antibody

Anti-Pirh2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117588
Anti-Pirh2 antibody used in Western Blot (WB). GTX117588

Cat. No. GTX117588

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all Pirh2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

30 kDa. ( Note )

Positive Control

NT2D1

Predict Reactivity

Mouse, Rat, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.88 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Pirh2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173822

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ring finger and CHY zinc finger domain containing 1 , ARNIP , CHIMP , PIRH2 , PRO1996 , RNF199 , ZCHY , ZNF363

Cellular Localization

Nucleus , Cytoplasm

Background

The protein encoded by this gene has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. This gene is oncogenic because loss of p53 function contributes directly to malignant tumor development. Transcription of this gene is regulated by p53. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Pirh2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX117588

GTX117588 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Pirh2(GTX117588) antibody at 1:500 dilution.

Anti-Pirh2 antibody used in Western Blot (WB). GTX117588

GTX117588 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
10% SDS PAGE
GTX117588 diluted at 1:1000

REFERENCE

Application Reference

Kim YJ et al. Cancer Lett 2019; 440-441 : 168-179 Primate-specific miR-944 activates p53-dependent tumor suppression in human colorectal cancers.
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169