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Placental Alkaline Phosphatase antibody [8F10-2C10-C9]

Anti-Placental Alkaline Phosphatase antibody [8F10-2C10-C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49147
Anti-Placental Alkaline Phosphatase antibody [8F10-2C10-C9] used in Western Blot (WB). GTX49147

Cat No. GTX49147

Host

Mouse

Clonality

Monoclonal

Clone Name

8F10-2C10-C9

Isotype

IgG2b

Application

WB, ICC/IF

Reactivity

Human
Package
100 μg ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide, 50% glycerol, 0.1%BSA

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human PLAP protein fragments expressed in E.coli.

Purification

Affinity purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Alkaline Phosphatase, Placental , Alp , Palp , Plap , Plap-1 , Alpp

Cellular Localization

Cell membrane; Lipid-anchor > GPI-anchor

Background

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2 while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme, also referred to as the heat stable form, that is expressed primarily in the placenta although it is closely related to the intestinal form of the enzyme as well as to the placental-like form. The coding sequence for this form of alkaline phosphatase is unique in that the 3´ untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2 and type 3) for this form of alkaline phosphatase have been well characterized.

Database

Research Area

DATA IMAGES

Anti-Placental Alkaline Phosphatase antibody [8F10-2C10-C9] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49147

GTX49147 ICC/IF Image

ICC/IF analysis of HeLa cells using PLAP antibody at a dilution of 1:300.

Anti-Placental Alkaline Phosphatase antibody [8F10-2C10-C9] used in Western Blot (WB). GTX49147

GTX49147 WB Image

WB analysis of LNCap, HeLa, human placenta and A549 lysates using PLAP antibody at a dilution of 1:500.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf