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Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™

Recombinant antibody HistoMAX
Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413
Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413
Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413

Cat. No. GTX04413

Host

Rabbit

Clonality

Monoclonal

Clone Name

MSVA-350R

Isotype

IgG

Application

IHC-P

Reactivity

Human
Package
500 μl ($679),
100 μl ($299)
PRODUCT

Summary

This antibody was validated on 76 different Normal Tissues by IHC-P.

Go to Normal Tissue GalleryGo to Cancer Tissue Gallery

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
IHC-P 1:100-1:200

Note :

IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.

This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.

Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Positive Control

Placenta (mature): A strong membranous and cytoplasmic staining of cytotrophoblast and syncytiotrophoblast must be seen.

Negative Control

Appendix: PLAP immunostaining must be absent in all structures.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA (Please contact us for PBS only format)

Preservative

0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human ALPP protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkaline phosphatase, placental , ALP , ALPI , IAP , PALP , PLAP , PLAP-1

Background

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413

GTX04413 IHC-P Image

IHC-P analysis of human placenta mature amnion and chorion tissue section using GTX04413 Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™.

Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413

GTX04413 IHC-P Image

IHC-P analysis of human testicular seminoma tissue section using GTX04413 Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™.
Strong Placental Alkaline Phosphatase Immunostaining in all cells of a testicular seminoma.

Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413

GTX04413 IHC-P Image

IHC-P analysis of human placenta tissue section using GTX04413 Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™.

REFERENCE

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REVIEW

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Package List Price ($)
$ 679
$ 299