PRODUCT
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:200 |
|
Note :
IHC-P
Manual staining : Heat-induced antigen retrieval for 5 minutes in an autoclave at 121ºC in pH 9.0 Tris-EDTA-based Target Retrieval Solution buffer. Based on testing result, pH 9.0 showed a strongest staining pattern. However, pH 9.0 results in only a slight reduction of the staining intensity as compared to pH 9.0. Therefore, the pH 7.8 was an optimal for manual staining because of the better tissue preservation at pH 7.8 than at pH 9.0.
This antibody has been validated by Autostainer including Agilent/Dako - Autostainer Link 48, Bond RX-Lecia, and Roche-Ventana Discovery ULTRA. The detailed steps by Autostainer please refer to the protocol.
|
Not tested in other applications.
Calculated MW
Positive Control
Placenta (mature): A strong membranous and cytoplasmic staining of cytotrophoblast and syncytiotrophoblast must be seen.
Negative Control
Appendix: PLAP immunostaining must be absent in all structures.
PROPERTIES
Form
Liquid
Buffer
10mM PBS, 0.05% BSA (Please contact us for sodium azide-free or sodium azide and BSA-free format)
Preservative
0.05% sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant full-length human ALPP protein
Purification
Protein A/G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
alkaline phosphatase, placental , ALP , ALPI , IAP , PALP , PLAP , PLAP-1
Background
The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]
Database
Research Area
DATA IMAGES
![Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413](/templates/default/js/lightbox/images/loading.gif "Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413")
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GTX04413 IHC-P Image
IHC-P analysis of human testicular seminoma tissue section using GTX04413 Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™.
Strong Placental Alkaline Phosphatase Immunostaining in all cells of a testicular seminoma.
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GTX04413 IHC-P Image
IHC-P analysis of human placenta tissue section using GTX04413 Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™.
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GTX04413 IHC-P Image
IHC-P analysis of human placenta mature amnion and chorion tissue section using GTX04413 Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™.
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REFERENCE
There are currently no references for Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ (GTX04413). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ (GTX04413). Be the first to share your experience with this product.
![Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413](/upload/website/prouct_img/small/GTX04413/GTX04413_20230728_IHC-P_358_23072723_324.webp "Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413")
![Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413](/upload/website/prouct_img/small/GTX04413/GTX04413_20230728_IHC-P_98_23072722_473.webp "Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413")
![Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413](/upload/website/prouct_img/small/GTX04413/GTX04413_20230728_IHC-P_228_23072723_906.webp "Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413")
![Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413](/upload/website/prouct_img/normal/GTX04413/GTX04413_20230728_IHC-P_358_23072723_324.webp "Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413")
![Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413](/upload/website/prouct_img/normal/GTX04413/GTX04413_20230728_IHC-P_98_23072722_473.webp "Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413")
![Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413](/upload/website/prouct_img/normal/GTX04413/GTX04413_20230728_IHC-P_228_23072723_906.webp "Anti-Placental Alkaline Phosphatase antibody [MSVA-350R] HistoMAX™ used in IHC (Paraffin sections) (IHC-P). GTX04413")