Placental Alkaline Phosphatase antibody [PL8-F6]

Cat. No. GTX34975

Host	Mouse
Clonality	Monoclonal
Clone Name	PL8-F6
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P, IHC-Fr, FACS
Reactivity	Human
Package	100 μg ($529)

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Datasheet
Document

View product citations for antibody GTX34975 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	0.25-0.5μg/ml for 30 minutes at RT
IHC-Fr	0.25-0.5μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells

Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Product Note

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified human PLAP protein

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ALP , ALPP , PALP , PLAP , PLAP1 , alkaline phosphatase, placental , Placental alkaline phosphatase

Cellular Localization

Cell membrane

Background

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]

Database

Gene ID: 250 ALPP
UniProt: P05187 ALPP

Research Area

DATA IMAGES

GTX34975 IHC-P Image

IHC-P analysis of human placenta tissue using GTX34975 Placental Alkaline Phosphatase antibody [PL8-F6].

GTX34975 WB Image

WB analysis of JEG-3 cell lysate using GTX34975 Placental Alkaline Phosphatase antibody [PL8-F6].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty