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Placental Alkaline Phosphatase antibody [PL8-F6]

Anti-Placental Alkaline Phosphatase antibody [PL8-F6] used in IHC (Paraffin sections) (IHC-P). GTX34975

Cat No. GTX34975

Host Mouse
Clonality Monoclonal
Clone Name PL8-F6
Isotype IgG2b
Application ICC/IF, IHC-P, IHC-Fr
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 0.5-1ug/ml
IHC-P 0.25-0.5ug/ml for 30 minutes at RT
IHC-Fr 0.25-0.5ug/ml for 30 minutes at RT
Not tested in other applications.

Calculated MW

58 kDa. ( Note )

Specificity/Sensitivity

Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.Ā
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified human PLAP protein

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Alkaline Phosphatase, Placental,Alp,Palp,Plap,Plap-1,Alpp

Background

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES
Anti-Placental Alkaline Phosphatase antibody [PL8-F6] used in IHC (Paraffin sections) (IHC-P). GTX34975

GTX34975 IHC-P Image

Formalin-fixed, paraffin-embedded human Placenta stained with PLAP Monoclonal Antibody (PL8-F6).

Package List Price ($)
$ 329