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Placental Alkaline Phosphatase antibody

Anti-Placental Alkaline Phosphatase antibody used in IHC (Frozen sections) (IHC-Fr). GTX72989

Cat. No. GTX72989

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr, IP

Reactivity

 Human, Rat
Package
500 μl ($399)
See all Placental Alkaline Phosphatase products

APPLICATION

Application Note

Immunoblot 1:100-1:500. Immunoprecipitation, Immunohistochemistry 1:50. The optimal conditions should be determined by the individual laboratory. This antibody can be used on formalin-fixed, paraffin-embedded tissue sections. Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 with AutoProbe III (GTX28620). It is recommended that this product be used on frozen tissue sections or specimens. The optimal conditions should be determined by the individual laboratory.

Calculated MW

58 kDa. ( Note )

Positive Control

Third Trimester Placenta

Product Note

Reacts with human PLAP specifically as determined by immunodiffusion in an agarose plate.

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 0.2% BSA

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified PLAP from human placenta

Purification

Protein A affinity purified
From polyclonal serum

Conjugation

Unconjugated

RRID

AB_374630

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

alkaline phosphatase, placental , ALP , ALPI , IAP , PALP , PLAP , PLAP-1

Background

The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid monoesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015]

Database

Research Area

DATA IMAGES

Anti-Placental Alkaline Phosphatase antibody used in IHC (Frozen sections) (IHC-Fr). GTX72989

GTX72989 IHC-Fr Image

The data was published in the journal Sci Rep in 2019.PMID: 31300685

REFERENCE

Application Reference

Kohno T et al. J Neurosci 2020; 40 (43) : 8248-8261 Reelin-Nrp1 Interaction Regulates Neocortical Dendrite Development in a Context-Specific Manner.
Satu? M et al. Sci Rep 2019; 9 (1) : 10153 Intra-articularly injected mesenchymal stem cells promote cartilage regeneration, but do not permanently engraft in distant organs.
Application : IHC-Fr
Reactivity : Human
Zwolanek D et al. JCI Insight 2017; 2 (20) Tracking mesenchymal stem cell contributions to regeneration in an immunocompetent cartilage regeneration model.
Application : IHC-Fr
Kohno T et al. J Neurosci 2015; 35 (11) : 4776-87 Importance of Reelin C-terminal region in the development and maintenance of the postnatal cerebral cortex and its regulation by specific proteolysis.
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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399