APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1-5μg/ml |
| 5-10μg/ml |
Not tested in other applications.
Calculated MW
Product Note
This antibody recognizes two variants of prekallikrein in human plasma, 88kDa and 85kDa, and its activation products kallikrein, the complexes formed by kallikrein with its endogenous inhibitors C1 inhibitor, alpha-2-macroglobulin and antithrombin III, and 45kDa prekallikrein/kallikrein fragments.The epitope for this antibody is located on the prekallikrein/kallikrein heavy chain and is involved in the interaction between prekallikrein and factor XIIa. This antibody inhibits prekallikrein activation in human and rhesus plasmas by ~60- 80% and 55%, respectively. This antibody does not cross-react with tissue kallikrein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Purified human prekallikrein.
Purification
Protein G purified
Conjugation
Unconjugated
RRID
AB_384595
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
KLK3 , KLKB1 , PKK , PKKD , PPK , kallikrein B1 , Plasma Kallikrein 1B , plasma prekallikrein , plasma kallikrein
Cellular Localization
Secreted
Background
This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
Database
Research Area
DATA IMAGES
![Anti-Plasma Kallikrein 1B antibody [13G11] used in Western Blot (WB). GTX21006](/templates/default/js/lightbox/images/loading.gif "Anti-Plasma Kallikrein 1B antibody [13G11] used in Western Blot (WB). GTX21006")
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GTX21006 WB Image
WB analysis of purified human prekallikrein protein (Lane 1) and normal human plasma (Lanes 2 and 3) lysates using GTX21006 Plasma Kallikrein 1B antibody [13G11].
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REFERENCE
REVIEW
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![Anti-Plasma Kallikrein 1B antibody [13G11] used in Western Blot (WB). GTX21006](/upload/website/prouct_img/small/GTX21006/GTX21006_20191119_WB_w_23060620_132.webp "Anti-Plasma Kallikrein 1B antibody [13G11] used in Western Blot (WB). GTX21006")
![Anti-Plasma Kallikrein 1B antibody [13G11] used in Western Blot (WB). GTX21006](/upload/website/prouct_img/normal/GTX21006/GTX21006_20191119_WB_w_23060620_132.webp "Anti-Plasma Kallikrein 1B antibody [13G11] used in Western Blot (WB). GTX21006")