APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500~1:1000 |
Not tested in other applications.
Calculated MW
Product Note
Plasma Kallikrein 1B (cleaved Arg390) antibody detects endogenous levels of fragment of activated KLKB1 resulting from cleavage adjacent to Arg390.
PROPERTIES
Form
Liquid
Buffer
PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
The antiserum was produced against synthesized peptide derived from human Plasma Kallikrein 1B (341-390).
Purification
Purified by antigen-affinity chromatography
From serum
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
KLK3 , KLKB1 , PKK , PKKD , PPK , kallikrein B1 , Plasma Kallikrein 1B , plasma prekallikrein , plasma kallikrein
Cellular Localization
Secreted
Background
Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
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GTX86891 WB Image
WB analysis of HeLa cell lysates using GTX86891 Plasma Kallikrein 1B (cleaved Arg390) antibody. The lane on the right is blocked with the synthesized peptide.
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REFERENCE
There are currently no references for Plasma Kallikrein 1B (cleaved Arg390) antibody (GTX86891). Be the first to share your publications with this product.
REVIEW
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