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Plasminogen antibody

Anti-Plasminogen antibody used in Western Blot (WB). GTX102877
Anti-Plasminogen antibody used in Western Blot (WB). GTX102877
Anti-Plasminogen antibody used in Western Blot (WB). GTX102877
Anti-Plasminogen antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102877
Anti-Plasminogen antibody used in Western Blot (WB). GTX102877
Anti-Plasminogen antibody used in Western Blot (WB). GTX102877
Anti-Plasminogen antibody used in IHC (Paraffin sections) (IHC-P). GTX102877
Anti-Plasminogen antibody used in IHC (Paraffin sections) (IHC-P). GTX102877
Anti-Plasminogen antibody used in Western Blot (WB). GTX102877
Anti-Plasminogen antibody used in IHC (Paraffin sections) (IHC-P). GTX102877

Cat. No. GTX102877

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Plasminogen products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , BCL-1 , PC-12 , Rat2 , mouse plasma , rat plasma , human plasma

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Plasminogen. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11168069

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

plasminogen

Cellular Localization

Secreted

Background

Plasminogen (PLG) is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between arg560 and val561, which is mediated by urokinase (PLAU; MIM 191840) and tissue plasminogen activator (PLAT; MIM 173370). The main function of plasmin is to dissolve fibrin (see, e.g., FGA, MIM 134820) clots. Plasmin, like trypsin, belongs to the family of serine proteinases (Miyata et al., 1982 [PubMed 6216475]; Forsgren et al., 1987 [PubMed 3030813]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-Plasminogen antibody used in Western Blot (WB). GTX102877

GTX102877 WB Image

Sample (30 μg of whole cell lysate)
A: Jurkat
B: Raji
C: K562
7.5% SDS PAGE
GTX102877 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Plasminogen antibody used in Western Blot (WB). GTX102877

GTX102877 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody (GTX102877) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Plasminogen antibody used in Western Blot (WB). GTX102877

GTX102877 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody (GTX102877) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Plasminogen antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102877

GTX102877 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Plasminogen(GTX102877) antibody at 1:500 dilution.

Anti-Plasminogen antibody used in Western Blot (WB). GTX102877

GTX102877 WB Image

Sample (30 μg of whole cell lysate)
A: BCL-1
7.5% SDS PAGE
GTX102877 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Plasminogen antibody used in Western Blot (WB). GTX102877

GTX102877 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody (GTX102877) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Plasminogen antibody used in IHC (Paraffin sections) (IHC-P). GTX102877

GTX102877 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Plasminogen(GTX102877) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Plasminogen antibody used in IHC (Paraffin sections) (IHC-P). GTX102877

GTX102877 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Plasminogen(GTX102877) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Plasminogen antibody used in Western Blot (WB). GTX102877

GTX102877 WB Image

Plasminogen antibody detects PLG protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
Plasminogen antibody (GTX102877) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Plasminogen antibody used in IHC (Paraffin sections) (IHC-P). GTX102877

GTX102877 IHC-P Image

Plasminogen antibody detects Plasminogen protein at cytoplasm in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
Plasminogen antibody (GTX102877) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Okada A et al. Sci Rep 2024; 14 (1) : 3105 C5a-C5AR1 axis as a potential trigger of the rupture of intracranial aneurysms.
Palmer OR et al. Res Pract Thromb Haemost 2018; 2 (2) : 266-273 Update on the electrolytic IVC model for pre-clinical studies of venous thrombosis.
Palmer O.R. et al. research and practice in thrombosis and haemostasis 2018; (Epub) Update on the electrolytic IVC model for pre-clinical studies of venous thrombosis.
Application : IHC-P
Reactivity : Mouse
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169