GeneTex
United States (US)

Plasminogen antibody

GTX102877_40730_WB_19011506_691.jpg
GTX102877_40730_20160204_WB_M_plasma_19011506_560.jpg
GTX102877_40730_20160204_WB_R_plasma_19011506_959.jpg
GTX102877_40730_WB_M_19011506_338.jpg
GTX102877_40730_IHC_M_19011506_660.jpg
GTX102877_40730_IFA_19011506_300.jpg
GTX102877_42508_20160707_WB_H_plasma_19011506_400.jpg
GTX102877_40730_20150703_IHC-P_R_19011506_941.jpg
GTX102877_40730_WB_R_19011506_183.jpg
GTX102877_40730_IHC_19011506_209.jpg

Cat No. GTX102877

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

Jurkat , Raji , K562 , BCL-1 , PC-12 , Rat2 , human plasma , mouse plasma , rat plasma

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.18 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Plasminogen. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Plasminogen,Plg

Cellular Localization

Secreted

Background

Plasminogen (PLG) is a circulating zymogen that is converted to the active enzyme plasmin by cleavage of the peptide bond between arg560 and val561, which is mediated by urokinase (PLAU; MIM 191840) and tissue plasminogen activator (PLAT; MIM 173370). The main function of plasmin is to dissolve fibrin (see, e.g., FGA, MIM 134820) clots. Plasmin, like trypsin, belongs to the family of serine proteinases (Miyata et al., 1982 [PubMed 6216475]; Forsgren et al., 1987 [PubMed 3030813]).[supplied by OMIM]

Database

Research Area

DATA IMAGES
GTX102877_40730_WB_19011506_691.jpg

GTX102877 WB Image

Sample (30 µg of whole cell lysate)
A: Jurkat
B: Raji
C: K562
7.5% SDS PAGE
GTX102877 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102877_40730_20160204_WB_M_plasma_19011506_560.jpg

GTX102877 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody (GTX102877) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102877_40730_20160204_WB_R_plasma_19011506_959.jpg

GTX102877 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody (GTX102877) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102877_40730_WB_M_19011506_338.jpg

GTX102877 WB Image

Sample (30 µg of whole cell lysate)
A: BCL-1
7.5% SDS PAGE
GTX102877 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102877_40730_IHC_M_19011506_660.jpg

GTX102877 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using Plasminogen(GTX102877) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102877_40730_IFA_19011506_300.jpg

GTX102877 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Plasminogen(GTX102877) antibody at 1:500 dilution.

GTX102877_42508_20160707_WB_H_plasma_19011506_400.jpg

GTX102877 WB Image

Human tissue extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Plasminogen antibody (GTX102877) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102877_40730_20150703_IHC-P_R_19011506_941.jpg

GTX102877 IHC-P Image

Plasminogen antibody detects Plasminogen protein at cytoplasm in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
Plasminogen antibody (GTX102877) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102877_40730_WB_R_19011506_183.jpg

GTX102877 WB Image

Plasminogen antibody detects PLG protein by western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
7.5% SDS-PAGE
Plasminogen antibody (GTX102877) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102877_40730_IHC_19011506_209.jpg

GTX102877 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human hepatoma, using Plasminogen(GTX102877) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169