GeneTex
United States (US)

Pleckstrin antibody [C2C3], C-term

GTX106332_39757_IFA_19011506_648.jpg
GTX106332_WB_19011506_907.jpg

Cat No. GTX106332

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

40 kDa. ( Note )

Positive Control

Raji

Predict Reactivity

Mouse, Rat, Bovine, Dog(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.61mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Pleckstrin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

FLJ27168 antibody, P47 antibody, PLEK antibody, platelet 47 kDa protein antibody, pleckstrin antibody

Background

Major protein kinase C substrate of platelets, its exact function is not known.

Database

DATA IMAGES
GTX106332_39757_IFA_19011506_648.jpg

GTX106332 ICC/IF Image

Pleckstrin antibody [C2C3], C-term detects PLEK protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in -20℃ 100% MeOH for 5 min.
Green: PLEK protein stained by Pleckstrin antibody [C2C3], C-term (GTX106332) diluted at 1:500.
Blue: Hoechst 33343 staining.

GTX106332_WB_19011506_907.jpg

GTX106332 WB Image

Sample(30 μg of whole cell lysate)
A:Raji(GTX27908)
12% SDS PAGE
GTX106332 diluted at 1:500

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169