GeneTex
United States (US)

Pleiotrophin antibody

Cat No. GTX10849

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB, ELISA
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.2 μg/ml
ELISA 1:200
Not tested in other applications.

Calculated MW

19 kDa. ( Note )

Positive Control

female Lewis rats cutaneous nerve protein
PROPERTIES

Form

Liquid

Buffer

PBS, 5% trehalose

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

purified recombinant human pleiotrophin expressed in Sf 21 cells.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Pleiotrophin,Harp,Hb-Gam,Hbbm,Hbgf-8,Hbgf8,Hbnf,Hbnf-1,Negf1,Osf-1,Ptn

Background

Human pleiotrophin is produced by a 168 amino acid residue PTN precursor. A 136 amino acid residue mature recombinant human pleiotrophin results from removal of the 32 amino acid residue signal peptide. Recombinant PTN has a predicted molecular mass of approximately 15.3 kDa. In SDS-PAGE, it migrates as an 18 kDa band, which may be due to its high lysine content. Pleiotrophin is highly conserved; human, bovine, rat, and mouse PTN share >98% amino acid sequence homology. Pleiotrophin is a member of a family of heparin-binding, developmentally regulated cytokines. It is active in growth and development and has mitogenic and neurite outgrowth activity. The gene for pleiotrophin is highly expressed in brain, uterus, gut, muscle,lung, and skin tissues. Pleiotrophin mRNA is expressed in osteoblasts, chondrocytes, fibroblasts, astrocytes, Schwann cells, and tumor cells.

Database

Research Area

Package List Price ($)
$ 289