PBS, 5% trehalose
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
purified recombinant human pleiotrophin expressed in Sf 21 cells.
Purified by affinity chromatography
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Human pleiotrophin is produced by a 168 amino acid residue PTN precursor. A 136 amino acid residue mature recombinant human pleiotrophin results from removal of the 32 amino acid residue signal peptide. Recombinant PTN has a predicted molecular mass of approximately 15.3 kDa. In SDS-PAGE, it migrates as an 18 kDa band, which may be due to its high lysine content. Pleiotrophin is highly conserved; human, bovine, rat, and mouse PTN share >98% amino acid sequence homology. Pleiotrophin is a member of a family of heparin-binding, developmentally regulated cytokines. It is active in growth and development and has mitogenic and neurite outgrowth activity. The gene for pleiotrophin is highly expressed in brain, uterus, gut, muscle,lung, and skin tissues. Pleiotrophin mRNA is expressed in osteoblasts, chondrocytes, fibroblasts, astrocytes, Schwann cells, and tumor cells.