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Podoplanin antibody [5E2]

Anti-Podoplanin antibody [5E2] used in Flow cytometry (FACS). GTX50043
Anti-Podoplanin antibody [5E2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50043

Cat No. GTX50043

Host

Mouse

Clonality

Monoclonal

Clone Name

5E2

Isotype

IgG2b

Application

ICC/IF, IHC-Fr, FACS, ELISA

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
ICC/IF 1:100
IHC-Fr Assay dependent
FACS 2-5 μg
ELISA Assay dependent

Note :

FACS
for 1x106 cells

Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Supplied in Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PDPN (Podoplanin) (1-206aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Podoplanin , Aggrus , Gp36 , Gp40 , Gp38 , Ht1A-1 , Ots8 , Pa2.26 , T1A , T1A-2 , T1A2 , Ti1A , Pdpn

Background

Podoplanin(PDPN) is a small mucin-like transmembrane protein, widely expressed in various specialized cell types throughout the body. This is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character.

Research Area

DATA IMAGES

Anti-Podoplanin antibody [5E2] used in Flow cytometry (FACS). GTX50043

GTX50043 FACS Image

FACS analysis of HeLa cells using GTX50043 PDPN antibody [5E2].
Red : PDPN
Dark gray : Isotype control
Light gray : Negative control, without primary and secondary antibody incubation

Anti-Podoplanin antibody [5E2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50043

GTX50043 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX50043 PDPN antibody [5E2].
Green : PDPN
Blue : cell nucleus
Dilution : 1:100

REFERENCE

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319