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Podoplanin antibody [5E2]

Anti-Podoplanin antibody [5E2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50043
Anti-Podoplanin antibody [5E2] used in Flow cytometry (FACS). GTX50043

Cat. No. GTX50043

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5E2

Isotype

 IgG2b

Application

 ICC/IF, IHC-P, IHC-Fr, FACS, ELISA

Reactivity

 Human, Mouse
Package
100 μl ($339)
See all Podoplanin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
ICC/IF 1:100
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS 2-5 μg
ELISA Assay dependent

Note :

FACS
for 1x106 cells
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PDPN (Podoplanin) (1-206aa) purified from E. coli

Purification

By protein-G affinity chromatography

Conjugation

Unconjugated

RRID

AB_11166297

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Podoplanin , Aggrus , Gp36 , Gp40 , Gp38 , Ht1A-1 , Ots8 , Pa2.26 , T1A , T1A-2 , T1A2 , Ti1A , Pdpn

Background

Podoplanin(PDPN) is a small mucin-like transmembrane protein, widely expressed in various specialized cell types throughout the body. This is a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character.

Research Area

DATA IMAGES

Anti-Podoplanin antibody [5E2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50043

GTX50043 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX50043 PDPN antibody [5E2].
Green : PDPN
Blue : cell nucleus
Dilution : 1:100

Anti-Podoplanin antibody [5E2] used in Flow cytometry (FACS). GTX50043

GTX50043 FACS Image

FACS analysis of HeLa cells using GTX50043 PDPN antibody [5E2].
Red : PDPN
Dark gray : Isotype control
Light gray : Negative control, without primary and secondary antibody incubation

REFERENCE

Application Reference

Oyama M et al. Evid Based Complement Alternat Med 2021; 2021 : 3707058 Saireito Improves Lymphatic Function and Prevents UVB-Induced Acute Inflammation and Photodamage in HR-1 Hairless Mice.
Application : IHC-P
Reactivity : Mouse
Narayan N et al. J Nucl Med 2018; (Epub) Translocator protein as an imaging marker of macrophage and stromal activation in RA pannus.
Application : IHC-Fr
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 339