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Podoplanin antibody [5E2]

Anti-Podoplanin antibody [5E2] used in IHC (Paraffin sections) (IHC-P). GTX85772
Anti-Podoplanin antibody [5E2] used in Western Blot (WB). GTX85772

Cat. No. GTX85772

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5E2

Isotype

 IgG2b

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse
Package
25 μg ($289)
See all Podoplanin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 5 μg/ml
ELISA Assay dependent

Note :

IHC-P
Heat induced antigen retrieval in pH 6.0 citrate buffer
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 10% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant human PDPN (1-206aa) purified from E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

podoplanin , AGGRUS , GP36 , GP40 , Gp38 , HT1A1 , OTS8 , PA2.26 , PDPN , T1A , T1A2 , TI1A , Podoplanin , gp36

Cellular Localization

Membrane,Cell projection,Filopodium membrane,Lamellipodium membrane,Microvillus membrane,Ruffle membrane

Background

This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Podoplanin antibody [5E2] used in IHC (Paraffin sections) (IHC-P). GTX85772

GTX85772 IHC-P Image

IHC-P analysis of human heart tissue using GTX85772 Podoplanin antibody [5E2].
Antigen retrieval : Heat-induced antigen retrieval
Dilution : 5 μg/ml

Anti-Podoplanin antibody [5E2] used in Western Blot (WB). GTX85772

GTX85772 WB Image

WB analysis of mouse kidney tissue lysate (50ug) using GTX85772 Podoplanin antibody [5E2].
Dilution : 1:500

REFERENCE

Clone Reference

Oyama M et al. Evid Based Complement Alternat Med 2021; 2021:3707058 Saireito Improves Lymphatic Function and Prevents UVB-Induced Acute Inflammation and Photodamage in HR-1 Hairless Mice.
Narayan N et al. J Nucl Med 2018; (Epub) Translocator protein as an imaging marker of macrophage and stromal activation in RA pannus.
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 289