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Podoplanin antibody [AT56F7]

Anti-Podoplanin antibody [AT56F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57668
Anti-Podoplanin antibody [AT56F7] used in Western Blot (WB). GTX57668

Cat No. GTX57668

Host

Mouse

Clonality

Monoclonal

Clone Name

AT56F7

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

Observed MW

35-42 kDa. The observed M.W. is based on the following publication. PMID: 22844530

PROPERTIES

Form

Liquid

Buffer

PBS (pH 7.4), 0.02% sodium azide, 10% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The clone AT56F7 is derived from hybridization of mouse F2 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PDPN protein.

Purification

Protein A Purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

podoplanin , AGGRUS , GP36 , GP40 , Gp38 , HT1A1 , OTS8 , PA2.26 , PDPN , T1A , T1A2 , TI1A , Podoplanin , gp36

Cellular Localization

Membrane,Cell projection,Filopodium membrane,Lamellipodium membrane,Microvillus membrane,Ruffle membrane

Background

This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Podoplanin antibody [AT56F7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX57668

GTX57668 ICC/IF Image

ICC/IF analysis of Hep3B cells using GTX57668 Podoplanin antibody.
Blue: DAPI
Green: Primary antibody
Dilution: 1:100

Anti-Podoplanin antibody [AT56F7] used in Western Blot (WB). GTX57668

GTX57668 WB Image

WB analysis of HeLa whole cell lysate using GTX57668 Podoplanin antibody.
Loading : 40 μg
Dilution : 1:1000

REFERENCE

There are currently no references for Podoplanin antibody [AT56F7] (GTX57668). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Podoplanin antibody [AT56F7] (GTX57668). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf