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Podoplanin antibody

Anti-Podoplanin antibody used in Western Blot (WB). GTX02422
Anti-Podoplanin antibody used in Immunoprecipitation (IP). GTX02422

Cat No. GTX02422

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IP

Reactivity

Human
Package
100 μl ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IP 1-2 μl/mg of lysate
Not tested in other applications.

Calculated MW

17 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.2 μm filtered PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant Human Podoplanin / PDPN Protein

Purification

Purified by Protein A and Antigen Affinity

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

podoplanin , AGGRUS , GP36 , GP40 , Gp38 , HT1A-1 , OTS8 , PA2.26 , T1A , T1A-2 , T1A2 , TI1A

Cellular Localization

Apical cell membrane

Background

This gene encodes a type-I integral membrane glycoprotein with diverse distribution in human tissues. The physiological function of this protein may be related to its mucin-type character. The homologous protein in other species has been described as a differentiation antigen and influenza-virus receptor. The specific function of this protein has not been determined but it has been proposed as a marker of lung injury. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Podoplanin antibody used in Western Blot (WB). GTX02422

GTX02422 WB Image

WB analysis of 293T whole cell lysate (Lane A) using GTX02422 Podoplanin antibody.
Dilution : 1:500
Loading : 30 μg

Anti-Podoplanin antibody used in Immunoprecipitation (IP). GTX02422

GTX02422 IP Image

IP analysis of 293T whole cell lysate using GTX02422 Podoplanin antibody.
IP antibody : 1 μl per 0.5 mg total whole cell lysate
Dilution : 1:500
50 % Protein G agarose : 15 μl

REFERENCE

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REVIEW

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