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Polycystin 2 antibody

Anti-Polycystin 2 antibody used in Western Blot (WB). GTX113802
Anti-Polycystin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113802
Anti-Polycystin 2 antibody used in Western Blot (WB). GTX113802

Cat No. GTX113802

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IHC-Wm

Reactivity

Human, Zebrafish
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

U87-MG

Predict Reactivity

Mouse, Rat, Bovine, Chicken, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Polycystin 2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11165095

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

polycystin 2, transient receptor potential cation channel , APKD2 , PC2 , PKD4 , Pc-2 , TRPP2

Cellular Localization

Membrane; Multi-pass membrane protein

Background

This gene encodes a member of the polycystin protein family. The encoded protein contains multiple transmembrane domains, and cytoplasmic N- and C-termini. The protein may be an integral membrane protein involved in cell-cell/matrix interactions. The encoded protein may function in renal tubular development, morphology, and function, and may modulate intracellular calcium homoeostasis and other signal transduction pathways. This protein interacts with polycystin 1 to produce cation-permeable currents. Mutations in this gene have been associated with autosomal dominant polycystic kidney disease. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Polycystin 2 antibody used in Western Blot (WB). GTX113802

GTX113802 WB Image

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX113802 diluted at 1:500.

Anti-Polycystin 2 antibody used in IHC (Paraffin sections) (IHC-P). GTX113802

GTX113802 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using Polycystin 2(GTX113802) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Polycystin 2 antibody used in Western Blot (WB). GTX113802

GTX113802 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Polycystin 2 antibody (GTX113802) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

REFERENCE

REVIEW

Polycystin 2 antibody Cat No. GTX113802

Rating ( Average 5 based on 1 users reviews)
Application
IHC (Whole mount)(IHC-Wm) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Figure Note : The data was peer reviewed and published in the journal Biol Open in 2015. PMID: 26432887
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169