Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using Polycystin 2(GTX113802) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: U87-MG
7.5% SDS PAGE
GTX113802 diluted at 1:500.

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Polycystin 2 antibody (GTX113802) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

The data was published in the journal Biol Open in 2015. PMID: 26432887