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GeneTex
United States (US)

Porcine circovirus type 2 / PCV2 Capsid antibody

GTX128121_41136_20160527_IHC-P_PCV2_18121410_281.jpg
GTX128121_41136_IHC_negative_18121410_227.jpg
GTX128121_41136_20160803_WB_B_18121410_899.jpg
GTX128121_41136_IHC_positive_18121410_121.jpg
GTX128121_41136_20160707_WB_PCV2_18121410_807.jpg

Cat No. GTX128121

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Porcine circovirus type 2
See all Porcine circovirus type 2 Capsid products
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

28 kDa. ( Note )

Positive Control

PCV2-infected PK-15 , PCV2 transfected
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Porcine circovirus type 2

Immunogen

Recombinant protein encompassing a sequence within the center region of Porcine circovirus type 2 Capsid protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ORF2

Background

The PCV2 virion measures approximately 17 nm in diameter, is non-enveloped and consists of a circular single-stranded DNA surrounded by an icosahedral capsid. The ambisense DNA molecule contains about 1.77 kb and 11 putative open reading frames (ORFs).
PCV2 has been associated with a number of disease syndromes in pigs. Post-weaning multisystemic wasting syndrome (PMWS) is an important emerging viral disease affecting nursery and grower pigs, which has recently been associated with porcine circovirus type 2 (PCV2) infections. PMWS has been reported in Europe, South East Asia, and the Americas.

Research Area

DATA IMAGES
GTX128121_41136_20160527_IHC-P_PCV2_18121410_281.jpg

GTX128121 IHC-P Image

Porcine circovirus type 2 Capsid antibody detects PCV2 on lymph node by immunohistochemical analysis.
Sample: Paraffin-embedded PCV2 free (left) and infected (right) swine lymph node.
Porcine circovirus type 2 Capsid antibody (GTX128121) dilution: 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX128121_41136_IHC_negative_18121410_227.jpg

GTX128121 IHC-P Image

Porcine circovirus type 2 Capsid antibody didn't detects PCV2 at macrophages on spleen by immunohistochemical analysis.
Sample: Paraffin-embedded PCV2 free swine spleen.
Porcine circovirus type 2 Capsid antibody (GTX128121) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX128121_41136_20160803_WB_B_18121410_899.jpg

GTX128121 WB Image

Lysate (30 µg) from Sf21 cells, expressing recombinant PCV2 protein by baculovirus system, was separated by 12% SDS-PAGE, and the membrane was blotted with Porcine circovirus type 2 Capsid antibody (GTX128121) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (The lysate was provided courtesy of Dr. Wu lab at Chung Yuan Christian University, Department of Bioscience Technology.)
Infected Sf21 whole cell extract provided from Dr. Wu lab, Chung Yuan Christian University, Department of Bioscience Technology.

GTX128121_41136_IHC_positive_18121410_121.jpg

GTX128121 IHC-P Image

Porcine circovirus type 2 Capsid antibody detects PCV2 at macrophages on spleen by immunohistochemical analysis.
Sample: Paraffin-embedded PCV2 infected swine spleen.
Porcine circovirus type 2 Capsid antibody (GTX128121) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
GTX128121_41136_20160707_WB_PCV2_18121410_807.jpg

GTX128121 WB Image

Infected (+) PK-15 whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with Porcine circovirus type 2 Capsid antibody (GTX128121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169