GeneTex
  • Country / Location Selection

United States (US)

Porcine circovirus type 2 / PCV2 replicase antibody

Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Western Blot (WB). GTX133638
Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133638

Cat No. GTX133638

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC

Reactivity

Porcine circovirus type 2
Package
100 μl ($319),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

PCV2-infected PK-15

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Porcine circovirus type 2

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of Porcine circovirus type 2 Replicase protein. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

DATA IMAGES

Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Western Blot (WB). GTX133638

GTX133638 WB Image

Non-infected (–) and infected (+) PK-15 whole cell extract (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Porcine circovirus type 2 ORF1 protein antibody (GTX133638) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Porcine circovirus type 2 / PCV2 replicase antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133638

GTX133638 ICC/IF Image

Porcine circovirus type 2 / PCV2 replicase antibody detects Porcine circovirus type 2 / PCV2 replicase protein by immunofluorescent analysis.
Sample: Uninfected and infected PK-15 cells were fixed in 4% paraformaldehyde at RT for 20 min.
Green: Porcine circovirus type 2 / PCV2 replicase stained by Porcine circovirus type 2 / PCV2 replicase antibody (GTX133638) diluted at 1:50.
Image courtesy of Dr. Karuppannan from Opriessnig Laboratory, Dept of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA.

REFERENCE

There are currently no references for Porcine circovirus type 2 / PCV2 replicase antibody (GTX133638). Be the first to share your publications with this product.

REVIEW

Porcine circovirus type 2 / PCV2 replicase antibody Cat No. GTX133638

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunohistochemistry(IHC) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-Sep-2019
Score :
Application Tested : WB
Sample Species : PCV2
Sample : PAM cells
Amount used : 30 μg
Blocking : 5 % non-fat milk, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Date : Anonymous submitted on 22-Jun-2018
Score :
Application Tested : IHC
Sample Species : PCV2
Sample : PK-15 cells
Blocking : 1% BSA in PBS with 0.05% Tween, 37°C, 1Hr
Fixation : 4% Paraformaldehyde in PBS, pH7.4, 25°C, 0.3Hr
Permeabilization /Antigen retrieval : 0.2% TritonX-100 in PBS, 25°C, 40 minHr
Primary Antibody : 1:50, 37°C, 1Hr
Figure Note : PCV2 non-infected (upper left) and infected (upper right, lower left and lower right) PK-15 cells. Image courtesy of Dr. Karuppannan from the lab "Opriessnig Laboratory, Dept of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, USA".
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf