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Pou5f3 (Oct4) antibody

Anti-Pou5f3 (Oct4) antibody used in Western Blot (WB). GTX132245

Cat. No. GTX132245

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Pou5f3 (Oct4) products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

2dpf zebrafish embryos

Predict Reactivity

Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of zebrafish Pou5f3 (Oct4). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886593

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

POU domain, class 5, transcription factor 3 , POU-2 , cb197 , chunp6868 , etID49452.21 , gp-9 , gp9 , oct4 , pou2 , pou5f1 , spg , spg/pou2 , wu:fd18d06

Cellular Localization

Nucleus

Background

Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t-POU2 causes developmental retardation or arrest before gastrulation.

Database

Research Area

DATA IMAGES

Anti-Pou5f3 (Oct4) antibody used in Western Blot (WB). GTX132245

GTX132245 WB Image

Zebrafish embryos extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pou5f3 antibody (GTX132245) diluted at 1:1000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169