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Pou5f3 (Oct4) antibody

Anti-Pou5f3 (Oct4) antibody used in Western Blot (WB). GTX132245

Cat No. GTX132245

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Zebrafish
Package
200 μl ($389),
50 μl ($199)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

Positive Control

2dpf zebrafish embryos
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.24 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of zebrafish Pou5f3 (Oct4). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

POU domain, class 5, transcription factor 3,POU-2,cb197,chunp6868,etID49452.21,gp-9,gp9,oct4,pou2,pou5f1,spg,spg/pou2,wu:fd18d06

Cellular Localization

Nucleus

Background

Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t-POU2 causes developmental retardation or arrest before gastrulation.

Database

Research Area

DATA IMAGES
Anti-Pou5f3 (Oct4) antibody used in Western Blot (WB). GTX132245

GTX132245 WB Image

Zebrafish embryos extract (30 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with Pou5f3 antibody (GTX132245) diluted at 1:1000.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199