Powassan virus NS2B antibody [HL4296]

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Powassan virus NS2B antibody [HL4296] (GTX643536) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Powassan virus NS2B antibody [HL4296] detects Powassan virus NS2B protein by immunofluorescent analysis.
Sample: Mock and POWV (MOI:1, 48 hr) infected A549 cells were fixed in 4 % formaldehyde and permeabilized using PBS containing 0.5 % Triton X-100 and 20 mM glycine.
Red: Powassan virus NS2B stained by Powassan virus NS2B antibody [HL4296] (GTX643536) diluted at 1:1000.
Blue: nuclear staining.