APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
Assay dependent |
Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No Preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Full lenngth human Prealbumin
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
transthyretin , ATTR , CTS , CTS1 , HEL111 , HsT2651 , PALB , TBPA
Cellular Localization
Secreted,Cytoplasm
Background
This gene encodes one of the three prealbumins, which include alpha-1-antitrypsin, transthyretin and orosomucoid. The encoded protein, transthyretin, is a homo-tetrameric carrier protein, which transports thyroid hormones in the plasma and cerebrospinal fluid. It is also involved in the transport of retinol (vitamin A) in the plasma by associating with retinol-binding protein. The protein may also be involved in other intracellular processes including proteolysis, nerve regeneration, autophagy and glucose homeostasis. Mutations in this gene are associated with amyloid deposition, predominantly affecting peripheral nerves or the heart, while a small percentage of the gene mutations are non-amyloidogenic. The mutations are implicated in the etiology of several diseases, including amyloidotic polyneuropathy, euthyroid hyperthyroxinaemia, amyloidotic vitreous opacities, cardiomyopathy, oculoleptomeningeal amyloidosis, meningocerebrovascular amyloidosis and carpal tunnel syndrome. [provided by RefSeq, Aug 2017]
Database
Research Area
DATA IMAGES
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GTX637548 IHC-P Image
Prealbumin antibody [HL1823] detects secreted Prealbumin protein by immunohistochemical analysis.Sample: Paraffin-embedded rat kidney.Prealbumin stained by Prealbumin antibody [HL1823] (GTX637548) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637548 IHC-P Image
Prealbumin antibody [HL1823] detects secreted Prealbumin protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.Prealbumin stained by Prealbumin antibody [HL1823] (GTX637548) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637548 WB Image
Human plasma (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Prealbumin antibody [HL1823] (GTX637548) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637548 ICC/IF Image
Prealbumin antibody [HL1823] detects Prealbumin protein by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Prealbumin stained by Prealbumin antibody [HL1823] (GTX637548) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
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GTX637548 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Prealbumin antibody [HL1823] (GTX637548) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637548 ICC/IF Image
Prealbumin antibody [HL1823] detects Prealbumin protein at cytoplasm by immunofluorescent analysis.Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Prealbumin stained by Prealbumin antibody [HL1823] (GTX637548) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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REFERENCE
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REVIEW
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