Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
2 - 4 μg/mL |
2 μg/mL |
Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Mouse Cerebellum Tissue Lysate(GTX24021) , Rat Brain Tissue Lysate(GTX27942)
Product Note
This antibody is specific for Precerebellin Internal
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Precerebellin antibody was raised against a 15 amino acid synthetic peptide from the middle of human precerebellin.The immunogen is located within amino acids 50 - 100 of Precerebellin.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
Synonyms
cerebellin 1 precursor
Cellular Localization
Secreted
Background
Precerebellin is the precursor of the brain-specific hexadecapeptide cerebellin, a protein with substantial similarity to the globular region of the B chain of complement component C1q (1). Cerebellin exerts neuromodulatory functions by directly stimulating norepinephrine release via the adenylate cyclase/pka- dependent signaling pathway (2); and indirectly enhances adrenocortical secretion in vivo, through a paracrine mechanism involving medullary catecholamine release (3). The active form of precerebellin is highly enriched in postsynaptic structures of cerebellar Purkinje cells in cartwheel neurons of the dorsal cochlear nucleus (4). Because precerebellin belongs to the C1q/tumor necrosis factor superfamily of secreted proteins and has similarity to adiponectin and CTRP3, it has been suggested that precerebellin posseses functions other than those already stated (5).
Database
Research Area