GeneTex
United States (US)

Presenilin 1 antibody [APS 11]

GTX15456_1014_IHC-P_19011519_250.jpg
GTX15456_1013_IHC-P_19011519_982.jpg
GTX15456_254_ICC-IF_19011519_574.jpg
GTX15456_1493_WB_19011519_282.jpg

Cat No. GTX15456

Host Mouse
Clonality Monoclonal
Clone Name APS 11
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP, ELISA
Reactivity Human, Mouse, Rat, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
ICC/IF 0.75 μg/ml
IHC-P 0.75 μg/ml
IP Assay dependent
ELISA 4.5 μg/ml
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Specificity/Sensitivity

No cross-reactivity is seen with presenilin 2. This antibody detects an ~28 kDa protein representing PS1 N-terminus cleavage product from ST15 cell lysate transfected with human PS1.
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues C H(21) L S N T V R S Q N D N R E(34) of human PS1.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Presenilin 1,Acninv3,Ad3,Fad,Ps-1,Ps1,S182,Psen1

Cellular Localization

Cell membrane,Cytoplasmic granule,Endoplasmic reticulum membrane,Golgi apparatus membrane

Background

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES
GTX15456_1014_IHC-P_19011519_250.jpg

GTX15456 IHC-P Image

IHC-P analysis of human liver tissue using GTX15456 Presenilin 1 antibody [APS 11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX15456_1013_IHC-P_19011519_982.jpg

GTX15456 IHC-P Image

IHC-P analysis of human brain tissue using GTX15456 Presenilin 1 antibody [APS 11].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX15456_254_ICC-IF_19011519_574.jpg

GTX15456 ICC/IF Image

ICC/IF analysis of mouse fibroblasts using GTX15456 Presenilin 1 antibody [APS 11].

GTX15456_1493_WB_19011519_282.jpg

GTX15456 WB Image

WB analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and mouse brain (lane 3) using GTX15456 Presenilin 1 antibody [APS 11].
Dilution : 1:200

Package List Price ($)
$ 319