APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:50 - 1:250 |
| 1:50 - 1:200 |
| 1:20 - 1:200 |
| Assay dependent |
| 1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
No cross-reactivity is seen with presenilin 2. This antibody detects an ~18 kDa protein representing PS1 CT (C-terminus fragment) and the ~46 kDa full-length PS1 from transfected SH-SY5Y cells.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide corresponding to residues C S(313) K Y N A E S T E R E S Q D T V A E N D D G(334) of humen PS1.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
presenilin 1 , ACNINV3 , AD3 , FAD , PS-1 , PS1 , S182
Cellular Localization
Endoplasmic reticulum,Endoplasmic reticulum membrane,Golgi apparatus membrane,Cell membrane
Background
Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq, Aug 2008]
Database
Research Area
DATA IMAGES
![Anti-Presenilin 1 antibody [APS 18] used in IHC (Paraffin sections) (IHC-P). GTX15458](/templates/default/js/lightbox/images/loading.gif "Anti-Presenilin 1 antibody [APS 18] used in IHC (Paraffin sections) (IHC-P). GTX15458")
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GTX15458 IHC-P Image
IHC-P analysis of human tonsil tissue using GTX15458 Presenilin 1 antibody [APS 18].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20
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GTX15458 ICC/IF Image
ICC/IF analysis of MCF-7 cells using GTX15458 Presenilin 1 antibody [APS 18]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
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GTX15458 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX15458 Presenilin 1 antibody [APS 18]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
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GTX15458 IHC-P Image
IHC-P analysis of human liver tissue using GTX15458 Presenilin 1 antibody [APS 18].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200
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GTX15458 ICC/IF Image
ICC/IF analysis of mouse fibroblasts using GTX15458 Presenilin 1 antibody [APS 18].
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GTX15458 ICC/IF Image
ICC/IF analysis of A2058 Cells using GTX15458 Presenilin 1 antibody [APS 18]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
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GTX15458 WB Image
WB analysis of 20 ug of THP-1 cell lysate using GTX15458 Presenilin 1 antibody [APS 18].
Dilution : 1:250
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REFERENCE
There are currently no references for Presenilin 1 antibody [APS 18] (GTX15458). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Presenilin 1 antibody [APS 18] (GTX15458). Be the first to share your experience with this product.
![Anti-Presenilin 1 antibody [APS 18] used in IHC (Paraffin sections) (IHC-P). GTX15458](/upload/website/prouct_img/small/GTX15458/GTX15458_1016_IHC-P_w_23060620_406.webp "Anti-Presenilin 1 antibody [APS 18] used in IHC (Paraffin sections) (IHC-P). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458](/upload/website/prouct_img/small/GTX15458/GTX15458_256_ICC-IF_w_23060620_123.webp "Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458](/upload/website/prouct_img/small/GTX15458/GTX15458_258_ICC-IF_w_23060620_479.webp "Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in IHC (Paraffin sections) (IHC-P). GTX15458](/upload/website/prouct_img/small/GTX15458/GTX15458_1015_IHC-P_w_23060620_767.webp "Anti-Presenilin 1 antibody [APS 18] used in IHC (Paraffin sections) (IHC-P). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458](/upload/website/prouct_img/small/GTX15458/GTX15458_255_ICC-IF_w_23060620_977.webp "Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458](/upload/website/prouct_img/small/GTX15458/GTX15458_257_ICC-IF_w_23060620_442.webp "Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in Western Blot (WB). GTX15458](/upload/website/prouct_img/small/GTX15458/GTX15458_1494_WB_w_23060620_951.webp "Anti-Presenilin 1 antibody [APS 18] used in Western Blot (WB). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in IHC (Paraffin sections) (IHC-P). GTX15458](/upload/website/prouct_img/normal/GTX15458/GTX15458_1016_IHC-P_w_23060620_406.webp "Anti-Presenilin 1 antibody [APS 18] used in IHC (Paraffin sections) (IHC-P). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458](/upload/website/prouct_img/normal/GTX15458/GTX15458_256_ICC-IF_w_23060620_123.webp "Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458](/upload/website/prouct_img/normal/GTX15458/GTX15458_258_ICC-IF_w_23060620_479.webp "Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in IHC (Paraffin sections) (IHC-P). GTX15458](/upload/website/prouct_img/normal/GTX15458/GTX15458_1015_IHC-P_w_23060620_767.webp "Anti-Presenilin 1 antibody [APS 18] used in IHC (Paraffin sections) (IHC-P). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458](/upload/website/prouct_img/normal/GTX15458/GTX15458_255_ICC-IF_w_23060620_977.webp "Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458](/upload/website/prouct_img/normal/GTX15458/GTX15458_257_ICC-IF_w_23060620_442.webp "Anti-Presenilin 1 antibody [APS 18] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15458")
![Anti-Presenilin 1 antibody [APS 18] used in Western Blot (WB). GTX15458](/upload/website/prouct_img/normal/GTX15458/GTX15458_1494_WB_w_23060620_951.webp "Anti-Presenilin 1 antibody [APS 18] used in Western Blot (WB). GTX15458")