GeneTex
United States (US)

Presenilin 1 antibody [APS 18]

GTX15458_256_ICC-IF_19011519_300.jpg
GTX15458_258_ICC-IF_19011519_355.jpg
GTX15458_255_ICC-IF_19011519_686.jpg
GTX15458_257_ICC-IF_19011519_543.jpg
GTX15458_1015_IHC-P_19011519_657.jpg
GTX15458_1494_WB_19011519_866.jpg
GTX15458_1016_IHC-P_19011519_713.jpg

Cat No. GTX15458

Host Mouse
Clonality Monoclonal
Clone Name APS 18
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP, ELISA, IHC
Reactivity Human, Mouse, Rat, Primate
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:50 - 1:250
ICC/IF 1:50 - 1:200
IHC-P 1:20 - 1:200
IP Assay dependent
ELISA 1:1000
IHC Assay dependent
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Specificity/Sensitivity

No cross-reactivity is seen with presenilin 2. This antibody detects an ~18 kDa protein representing PS1 CT (C-terminus fragment) and the ~46 kDa full-length PS1 from transfected SH-SY5Y cells.
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues C S(313) K Y N A E S T E R E S Q D T V A E N D D G(334) of humen PS1.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Presenilin 1,Acninv3,Ad3,Fad,Ps-1,Ps1,S182,Psen1

Cellular Localization

Cell membrane,Cytoplasmic granule,Endoplasmic reticulum membrane,Golgi apparatus membrane

Background

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq, Aug 2008]

Database

Research Area

DATA IMAGES
GTX15458_256_ICC-IF_19011519_300.jpg

GTX15458 ICC/IF Image

ICC/IF analysis of MCF-7 cells using GTX15458 Presenilin 1 antibody [APS 18]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 °C

GTX15458_258_ICC-IF_19011519_355.jpg

GTX15458 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15458 Presenilin 1 antibody [APS 18]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 °C

GTX15458_255_ICC-IF_19011519_686.jpg

GTX15458 ICC/IF Image

ICC/IF analysis of mouse fibroblasts using GTX15458 Presenilin 1 antibody [APS 18].

GTX15458_257_ICC-IF_19011519_543.jpg

GTX15458 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX15458 Presenilin 1 antibody [APS 18]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 °C

GTX15458_1015_IHC-P_19011519_657.jpg

GTX15458 IHC-P Image

IHC-P analysis of human liver tissue using GTX15458 Presenilin 1 antibody [APS 18].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

GTX15458_1494_WB_19011519_866.jpg

GTX15458 WB Image

WB analysis of 20 ug of THP-1 cell lysate using GTX15458 Presenilin 1 antibody [APS 18].
Dilution : 1:250

GTX15458_1016_IHC-P_19011519_713.jpg

GTX15458 IHC-P Image

IHC-P analysis of human tonsil tissue using GTX15458 Presenilin 1 antibody [APS 18].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

Package List Price ($)
$ 319