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Presenilin 1 antibody [C1C2], Internal

Anti-Presenilin 1 antibody [C1C2], Internal used in Western Blot (WB). GTX116016
Anti-Presenilin 1 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116016
Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016
Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016
Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016

Cat No. GTX116016

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Presenilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037782

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

presenilin 1 , ACNINV3 , AD3 , FAD , PS-1 , PS1 , S182

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein , Golgi apparatus membrane; Multi-pass membrane protein , Cell surface

Background

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Presenilin 1 antibody [C1C2], Internal used in Western Blot (WB). GTX116016

GTX116016 WB Image

Wild-type (WT) and Presenilin 1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Presenilin 1 antibody [C1C2], Internal (GTX116016) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Presenilin 1 antibody [C1C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116016

GTX116016 ICC/IF Image

Presenilin 1 antibody [C1C2], Internal detects Presenilin 1 protein at Golgi apparatus and endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: Presenilin 1 stained by Presenilin 1 antibody [C1C2], Internal (GTX116016) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016

GTX116016 IHC-P Image

Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Presenilin 1 antibody [C1C2], Internal (GTX116016) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016

GTX116016 IHC-P Image

Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Presenilin 1 antibody [C1C2], Internal (GTX116016) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Presenilin 1 antibody [C1C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX116016

GTX116016 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Presenilin 1(GTX116016) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

There are currently no references for Presenilin 1 antibody [C1C2], Internal (GTX116016). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Presenilin 1 antibody [C1C2], Internal (GTX116016). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169