GeneTex
United States (US)

Presenilin 1 antibody [C1C2], Internal

GTX116016_40135_IHC_M_18121410_824.jpg
GTX116016_40135_IHC_2_18121410_151.jpg
GTX116016_IHC_18121410_106.jpg
GTX116016_WB_18121410_259.jpg
GTX116016_40135_IFA_18121410_445.jpg

Cat No. GTX116016

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

0.53mg/ml

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Presenilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AD3 antibody, FAD antibody, PS1 antibody, S182 antibody, PSEN1 antibody, presenilin-1 antibody, presenilin 1 antibody

Cellular Localization

Endoplasmic reticulum membrane; Multi-pass membrane protein , Golgi apparatus membrane; Multi-pass membrane protein , Cell surface

Background

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq]
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Database

Research Area

DATA IMAGES
GTX116016_40135_IHC_M_18121410_824.jpg

GTX116016 IHC-P Image

Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Presenilin 1 antibody [C1C2], Internal (GTX116016) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116016_40135_IHC_2_18121410_151.jpg

GTX116016 IHC-P Image

Presenilin 1 antibody [C1C2], Internal detects PSEN1 protein at cytosol on U87 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U87 xenograft.
Presenilin 1 antibody [C1C2], Internal (GTX116016) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116016_IHC_18121410_106.jpg

GTX116016 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using Presenilin 1(GTX116016) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX116016_WB_18121410_259.jpg

GTX116016 WB Image

Sample (30 µg of whole cell lysate)
A: HeLa
10% SDS PAGE
GTX116016 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX116016_40135_IFA_18121410_445.jpg

GTX116016 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Presenilin 1(GTX116016) antibody at 1:200 dilution.

SDS
Glycerol.pdf
Thimerosal.pdf