GeneTex
United States (US)

Presenilin 1 antibody [C1C3]

GTX101028_39925_IHC_19011506_505.jpg
GTX101028_39925_IHC_2_19011506_761.jpg
GTX101028_39925_20160215_IHC-P_R_2_19011506_329.jpg
GTX101028_WB_M_19011506_318.jpg
GTX101028_39925_WB_19011506_626.jpg
GTX101028_39925_20160215_IHC-P_R_1_19011506_956.jpg
GTX101028_39925_IHC_M_19011506_552.jpg

Cat No. GTX101028

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

53 kDa. ( Note )

Positive Control

293T , A431 , Jurkat , Raji , NIH-3T3

Predict Reactivity

Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.29mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Presenilin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AD3 antibody, FAD antibody, PS1 antibody, S182 antibody, PSEN1 antibody, presenilin-1 antibody, presenilin 1 antibody

Background

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101028_39925_IHC_19011506_505.jpg

GTX101028 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Presenilin 1(GTX101028) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101028_39925_IHC_2_19011506_761.jpg

GTX101028 IHC-P Image

Presenilin 1 antibody [C1C3] detects PSEN1 protein at cytosol on U373 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U373 xenograft.
Presenilin 1 antibody [C1C3] (GTX101028) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101028_39925_20160215_IHC-P_R_2_19011506_329.jpg

GTX101028 IHC-P Image

Presenilin 1 antibody [C1C3] detects Presenilin 1 protein at cell membrane and cytoplasm in rat adrenal gland by immunohistochemical analysis.
Sample: Paraffin-embedded rat adrenal gland.
Presenilin 1 antibody [C1C3] (GTX101028) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101028_WB_M_19011506_318.jpg

GTX101028 WB Image

Sample (30 ug of whole cell lysate)

A:NIH-3T3
10% SDS PAGE

GTX101028 diluted at 1:1000

GTX101028_39925_WB_19011506_626.jpg

GTX101028 WB Image

Sample (30 ug of whole cell lysate)
A: Raji
10% SDS PAGE
GTX101028 diluted at 1:1000

GTX101028_39925_20160215_IHC-P_R_1_19011506_956.jpg

GTX101028 IHC-P Image

Presenilin 1 antibody [C1C3] detects Presenilin 1 protein at cell membrane and cytoplasm in rat lung by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
Presenilin 1 antibody [C1C3] (GTX101028) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101028_39925_IHC_M_19011506_552.jpg

GTX101028 IHC-P Image

Presenilin 1 antibody [C1C3] detects PSEN1 protein at cytosol on mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
Presenilin 1 antibody [C1C3] (GTX101028) dilution: 1:200.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf