APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:100-1:1000 |
| 0.75 μg/ml |
| 1:100 |
| 4.5 μg/ml |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
No cross-reactivity is seen with presenilin 1. This antibody detects an ~28 kDa protein representing PS2 NT (N-terminus fragment) and the ~46 kDa full-length PS2 from transfected COS-7 cells.
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide corresponding to residues C(31) Q E G R Q G P E D G E N T A(45) of human PS2.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
presenilin 2 , AD3L , AD4 , CMD1V , PS2 , STM2
Cellular Localization
Endoplasmic reticulum membrane,Golgi apparatus membrane
Background
Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-Presenilin 2 antibody [APS 21] used in IHC (Paraffin sections) (IHC-P). GTX15548](/templates/default/js/lightbox/images/loading.gif "Anti-Presenilin 2 antibody [APS 21] used in IHC (Paraffin sections) (IHC-P). GTX15548")
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GTX15548 IHC-P Image
IHC-P analysis of human kidney tissue using GTX15548 Presenilin 2 antibody [APS 21].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:100
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GTX15548 WB Image
WB analysis of 25 ug of SH-SY5Y (lane 1), human brain (lane 2) and PC12 (lane 3) using GTX15548 Presenilin 2 antibody [APS 21].
Dilution : 1:200
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GTX15548 ICC/IF Image
ICC/IF analysis of A2058 Cells using GTX15548 Presenilin 2 antibody [APS 21]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
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GTX15548 ICC/IF Image
ICC/IF analysis of NCI-H460 Cells using GTX15548 Presenilin 2 antibody [APS 21]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
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GTX15548 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX15548 Presenilin 2 antibody [APS 21]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:100 overnight at 4 ºC
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REFERENCE
There are currently no references for Presenilin 2 antibody [APS 21] (GTX15548). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for Presenilin 2 antibody [APS 21] (GTX15548). Be the first to share your experience with this product.
![Anti-Presenilin 2 antibody [APS 21] used in IHC (Paraffin sections) (IHC-P). GTX15548](/upload/website/prouct_img/small/GTX15548/GTX15548_1017_IHC-P_w_23060620_161.webp "Anti-Presenilin 2 antibody [APS 21] used in IHC (Paraffin sections) (IHC-P). GTX15548")
![Anti-Presenilin 2 antibody [APS 21] used in Western Blot (WB). GTX15548](/upload/website/prouct_img/small/GTX15548/GTX15548_1495_WB_w_23060620_115.webp "Anti-Presenilin 2 antibody [APS 21] used in Western Blot (WB). GTX15548")
![Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548](/upload/website/prouct_img/small/GTX15548/GTX15548_259_ICC-IF_w_23060620_351.webp "Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548")
![Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548](/upload/website/prouct_img/small/GTX15548/GTX15548_261_ICC-IF_w_23060620_497.webp "Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548")
![Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548](/upload/website/prouct_img/small/GTX15548/GTX15548_260_ICC-IF_w_23060620_893.webp "Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548")
![Anti-Presenilin 2 antibody [APS 21] used in IHC (Paraffin sections) (IHC-P). GTX15548](/upload/website/prouct_img/normal/GTX15548/GTX15548_1017_IHC-P_w_23060620_161.webp "Anti-Presenilin 2 antibody [APS 21] used in IHC (Paraffin sections) (IHC-P). GTX15548")
![Anti-Presenilin 2 antibody [APS 21] used in Western Blot (WB). GTX15548](/upload/website/prouct_img/normal/GTX15548/GTX15548_1495_WB_w_23060620_115.webp "Anti-Presenilin 2 antibody [APS 21] used in Western Blot (WB). GTX15548")
![Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548](/upload/website/prouct_img/normal/GTX15548/GTX15548_259_ICC-IF_w_23060620_351.webp "Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548")
![Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548](/upload/website/prouct_img/normal/GTX15548/GTX15548_261_ICC-IF_w_23060620_497.webp "Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548")
![Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548](/upload/website/prouct_img/normal/GTX15548/GTX15548_260_ICC-IF_w_23060620_893.webp "Anti-Presenilin 2 antibody [APS 21] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15548")