GeneTex
United States (US)

Presenilin 2 antibody [APS 26]

GTX15549_262_ICC-IF_19011519_370.jpg
GTX15549_263_ICC-IF_19011519_163.jpg
GTX15549_1018_IHC-P_19011519_380.jpg
GTX15549_264_ICC-IF_19011519_667.jpg
GTX15549_1496_WB_19011519_609.jpg

Cat No. GTX15549

Host Mouse
Clonality Monoclonal
Clone Name APS 26
Isotype IgG1
Application WB, ICC/IF, IHC-P, IP, ELISA, IHC
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:100-1:1000
ICC/IF 0.75 μg/ml
IHC-P 1:20
IP Assay dependent
ELISA 4.5 μg/ml
IHC Assay dependent
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Specificity/Sensitivity

No cross-reactivity is seen with presenilin 1. This antibody detects an ~20 kDa protein representing PS2 CT (C-terminal fragment) and the ~45 kDa full-length PS2 from transfected COS-7 cells.
PROPERTIES

Form

Liquid

Buffer

PBS, 1mg/ml BSA, 0.05% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.)

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to residues C L(317) P Y D P E M E E D S Y D S F G E P(334) of human PS2.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

AD3L,AD4,CMD1V,PS2,STM2

Cellular Localization

Endoplasmic reticulum membrane,Golgi apparatus membrane

Background

Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1 or PSEN2) or the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor such that, they either directly regulate gamma-secretase activity, or themselves act are protease enzymes. Two alternatively spliced transcript variants encoding different isoforms of PSEN2 have been identified. [provided by RefSeq, Jul 2008]
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Database

Research Area

DATA IMAGES
GTX15549_262_ICC-IF_19011519_370.jpg

GTX15549 ICC/IF Image

ICC/IF analysis of mouse fibroblasts using GTX15549 Presenilin 2 antibody [APS 26].

GTX15549_263_ICC-IF_19011519_163.jpg

GTX15549 ICC/IF Image

ICC/IF analysis of A2058 Cells using GTX15549 Presenilin 2 antibody [APS 26]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4°C

GTX15549_1018_IHC-P_19011519_380.jpg

GTX15549 IHC-P Image

IHC-P analysis of human kidney tissue using GTX15549 Presenilin 2 antibody [APS 26].
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:20

GTX15549_264_ICC-IF_19011519_667.jpg

GTX15549 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX15549 Presenilin 2 antibody [APS 26]. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4°C

GTX15549_1496_WB_19011519_609.jpg

GTX15549 WB Image

WB analysis of 25 ug of human brain (lane 1) and L929 (lane 2) using GTX15549 Presenilin 2 antibody [APS 26].
Dilution : 1:200